Open Access
Subscription or Fee Access
The Aqueous Solution of Antibiotics Norfloxacin for Total Protein Contents in the Fifth Instar Larvae of Silkworm, Bombyx mori (L) (Double Hybrid Race) [(CSR2XCSR27)] x [(CSR6XCSR26)].
Abstract
The quality of food material supplied decide the quality of silky cocoon spun by the larval instars of silkworm, Bombyx mori (L). The present attempt deals with utilization of aqueous solution of Norfloxacin for treating the leaves of mulberry, Morus alba (L). Two concentrations of Norfloxacin were selected for the attempt. Two concentrations of Norfloxacin include 50 mg per liter (50 ppm) and 100 mg per liter (100 ppm). The solution was prepared by adding powder antibiotic in distilled water and made the required concentration. 400 ml of aqueous solution of Norfloxacin were used to treat 100 grams of leaves of mulberry, Morus alba (L) for feeding the group of hundred larval instars for each attempt of feeding. Treated mulberry leaves were fed to the fifth larval instars. The feeding the larvae with treated leaves was carried for first four days. Four feedings were given for each day. For each feeding hundred grams of leaves (for the group of hundred larvae) were used. The larvae fed with untreated control & water treated control leaves were also maintained. Through the use of silk glands; fat bodies and haemolymph, bioassay of total protein was carried out on fifth day. The total protein content of silk glands; fat bodies and haemolymph was found improved 61.519 to 114.667; 79.928 to 90.055 and 30.983 to 31.010 percents respectively through feeding the fifth instars of silkworm, Bombyx mori (L) (Double Hybrid Race) leaves of mulberry, Morus alba (L) treated with aqueous solution of Norfloxacin antibiotics.
Keywords
References
Bowers W.S., Fales V.M., Thompson M.J. and Uebel B., Juvenile and gonadotropic activity of 10, 11 epoxyfranesoic acid methyl ester, Life Science,, 2323-2331 (1966)
Ito, H. (1960). Effect of sugars on feeding the larvae of silkworm, Bombyx mori (L) J. Insect. Physiol. Vol.5: 95-107.
Ito, T. (1961). Nutrition of silkworm, Bombyx mori (L). Proc. Jpn. Acad. Sci. 43:57-61.
Nayar, J.K.&Frankel,G.(1962). Journal of Insect Physiology. Volume-8, page-505.
Ito, T; Kwashima, K.; Nakhara,M.; Nakanshi, K. & Terahara, A. (1964). Metabolism in the mid gut of silkworm, Bombyx mori (L). Insect Physiol. Vol.10: 225-228.
Slansky,F. & Scriber, J.M. (1985). Food consumption & Utilization. In: comprehensive Insect physiology, Biochemistry & pharmacology. (Eds. Kerkut, G.A. and Gilbert, L.I.) 4, Pergamon Press, Oxford, Page: 639.
Horie, Y.; Tanaka,M. and Ito,T. (1963). Proteolytic enzyme of digestive juice of mid gut in silkworm, Bombyx mori (L). J. Setricult. Sci. Japan, 32: 8-15.
Horie, Y.(1961). Physiological studies on the alimentary canal of silkworm, Bombyxmori (L).III. Absorption& utilization of carbohydrates. Bull. Sericult. Exp. Sta. Japan, 16: 287-309.
Yamafuji, I. and Yonezawa (1935). Lipases in silkworm, Bombyx mori (L).Insect. Biochem. 1: 102-112.
Vitthalrao B. Khyade & Jyoti Kulkarni (2011). Effect of digoxin treated mulberry leaves on protein profiles in fifth instar larvae of silkworm, Bombyx mori (L) (PM x CSR2). Res. J. Chem. Sci. Vol.1 (1): 2-7 (www.isca.in)
Desai, V.A.; Pawar, V.V. &Sawant, R.T.(2011). Influence of herbal drug: kho-go on the fifth instar larvae of silkworm, Bombyx mori (L).Dissertation in the partial fulfillment of M.Sc. (Microbiology), Shardabai Pawar Mahila College, Shardanagar (Baramati), (Pune University, Pune).
Manisha Mahendra Nalwade, Kajal Appasaheb Pondkule, Vitthalrao B. Khyade (2018). The reflection of feeding the mulberry leaves treated with water solution of seed powder of Syzigium cumini (L) into Profiles of Protein (Total) in the fifth instar larvae of silk worm, Bombyx mori (L) Race - bivoltine, crossbreed: [(CSR6 x CSR26)] x [CSR2 x CSR27)]. International Journal of Scientific Research in Chemistry (IJSRCH) | Online ISSN: 2456-8457 © 2018 IJSRCH | Volume 3 | Issue 4:68– 76. http://ijsrch.com/archive.php?v=3&i=6&pyear=2018
Vitthalrao Bhimasha Khyade and Eric Richard Kandel (2018). Influence of Aqueous Solution of Eurhodin Treated Mulberry Leaves on the Quality of Cocoons and Silk Filament in Silkworm, Bombyx mori (L) Races: Bivoltine Crossbreed [(CSR6 x CSR26) x (CSR2 x CSR27)] and multivoltine crossbreed [(PM x CSR2)]. International Journal of Research in Science and Engineering Vol. 6, No. 4, 2018, pp. 22-37. ISSN 2347-9353. https://scientificrc.com/journals/international-journal-of-research-in-science-and-engineering/volume-6-issue-4-october-december-2018/
Sharad Ganpat Jagtap and Vitthalrao Bhimasha Khyade (2019). Influence of Treating Mulberry Leaves With Aqueous Solution of 3 – Amino – 7 – dimethylamino – 2 – methylphenazine hydrochloride Before feeding on the Silk Production in Silkworm, Bombyx mori (L). International Journal of Zoology and Applied Biosiences Vol. 4 Issue 1: 37 – 45. ISSN: 2455 – 9571.
Pragati Prabhakar Doke, Mansi Ramesh Das, Samiksha Sunil Pisal, Amruta Chandrakant Nimbalkar, Vitthalrao B. Khyade (2019). The Contents of Total Protein in the Fifth Instar Larvae of Silk Worm, Bombyx mori (L) fed with Mulberry Leaves Treated with Water Solution of Eurhodin. International Journal of Scientific Research in Chemistry (IJSRCH) © 2019 IJSRCH | Volume 4 | Issue 2 | ISSN : 2456-8457 www.ijsrch.com
Baig M, Nataraju B, Samson MV. Studies on the effect of antibiotics on rearing performance and loss due to diseases in silkworm, Bombyx mori L. Indian Journal of Sericulture. 1990;29(1):54-58.
Murthy MRV, Shankarappa BS, Sreenivasaya M. Influence of antibiotics on the growth of silkworm, Bombyx mori L. Current Science. 1951; 20: 269-270.
Murthy MRV, Sreenivasaya M. Effect of antibiotic onthe growth of silkworm, Bombyx mori L. Nature. 1954; 172: 684-685.
Shyamala MB, Maya G, Bhat JV. Chlormycetin in the nutrition of silkworm Bombyx mori L. influence of digestion and utilization of protein, fat and minerals. Indian Journal of Sericulture.1962; 1: 21-26.
Verma AN, Atwal AS. Effect of chloromycetin, glycine, and molasses on the growth of and production of silk by Bombyx mori L. (Lepidoptera: Bombycidae).Indian Journal of Sericulture. 1963; 2(4):1-4.
Ahmed Aftab, C.A (1994). Effect of diseased mulberry leaves Morus alba on food and water utilization in silkworm Bombyx mori (L.).Ph. D. Thesis, Bangalore University, p.151.
Shiva kumar,C (1995) Physiological and biochemical studies on nutrition in silkworm, Bombyx mori L. Ph. D. Thesis Bangalore, University, Bangalore India.
Tayade DS, Jawale MD, Unchegaonkar PK. Effects of antibiotics on the growth of silkworm, Bombyx mori L. Indian Journal of Sericulture. 1988; 27(2):69-72.
Rai BR, Devaiah MC. Effect of antibiotics on the incidence of disease in silkworm, Bombyx mori. L. International Congress on Tropical Sericulture Practices, Bangalore. India. 1988, 1-8.
Nataraju B, Sathyaprasad K, Manjunath D, Kumar CA. Silkworm crop protection, Central Silk Board, 2005, 61-85.
Babu, Mahesh S.,Gopalaswamy, G. and Chandramohan, N. (2005) Identification of an antiviral principle in Spirulina platensis against Bombyx mori nuclear polyhedrosis virus (bmnpv), Indian Journal of Biotechnology,.4, pp. 384-388.
Murugan, K.and George, A. (Sr.)(1992). Feedings & nutritional influence on growth & reproduction of Daphnia near (L). Insect Physiol. 38: 961-969.
Murali, K.(1992).Effect of leaf carbohydrate reserves on the growth& excretory pattern of silkworm, Bombyx mori (L). M.Phil. Dissertation, Sri. Venkateshwara University, Tirupati (India).
Subramanyam Reddy, C.(1992).Studies on distribution of digestive enzymes in the digestive tract of silkworm, Bombyx mori(L). M.Phil. Dissertation, Sri. Venkateshwara University, Tirupati (India).
Seema K. Dongare; Priti M. Gaikwad; Shubhangi S. Pawar and Vitthalrao B. Khyade (2019). The influence of infection of Beauveria bassiana (Bals) Vuill, a fungal species (Family: Clavicipitaceae) on quality of the cocoons of spinned by the larval instars of Bombyx mori (L) (Race: PMx CSR2 ). Journal of Bacteriology & Mycology. Int Phys Med Rehab J. 2019;7(1):14‒18. DOI: 10.15406/jbmoa.2019.07.00234
Chandrasekharan, K. and Nataraju, B. (2006) First report on the incidence of white muscardine disease caused by Beauveria bassiana, in uzi fly, Exorista bombycis, and its significance in sericulture, Indian journal of sericulture,.45 (1).
Hamamoto, Hiroshi, Kurakawa, Kenji, Chikara, Kaito, Kamura, Koushirou, Razanajatovo, Manintra, Iony, Kusuhara, Hiroyuki, Santa, Tomofumi and Sekimizu, Kazuhisa (2004) Quantitative evaluation of the therapeutic effects of antibiotics using silkworm infected with human pathogenic microorganisms, Antimicrobial Agents and Chemotherapy, 48(3), 774-779.
Kaito, C., Akimitsu, N., Watanabe, H. and Sekimizu, K. (2002). Silkworm larvae as an animal model of bacterial infection pathogenic to humans, Microbial Pathogenesis, 32(4), 183-90.
Orihara Yutaka, Hamamoto, Hiroshi, Kasuga, Hiroshi, Shimada, Toru, Kawaguchi, Yasushi and Sekimizu, Kazuhisa (2008) A Silkworm-Baculovirus model for assessing the therapeutic effects of antiviral Compounds: Characterization and application to the isolation of antivirals from traditional medicines, J. Gen Virol, 89, 188-194.
Krishnaswami, S.; Narasimhna, M.N.; Suryanarayana, S.K. and Kumararaj, S.(1978). Sericulture Manual-II Silkworm Rearing: FAO. United Nations Rome.
Khyade, V.B.(2004). Influence of juvenoids on silkworm, Bombyx mori (L). Ph.D. thesis, Shivaji University, Kolhapur.
Ramprakash Verma; Ravindra V. Kshirsagar and *Vitthalrao B. Khyade (2018). Treating the Leaves of Mulberry, Morus alba (L) (V -1 : Variety) with Aqueous Solution of Of Maple Syrup Belong To Acer Saccharum (L) And Feeding the Fifth Instar Larvae of Silkworm, Bombyx Mori (L) (Race: Bivoltine Double Hybrid [(Po3 × Nd5) × (Csr4 × Csr2)] For the Qualitative Silk Improvement. Asian Journal of Science and Technology Vol. 09, Issue, 03, pp.7708-7713, March, 2018 http://journalajst.com
Pranita Rajendra Vare., Seema Karna Dongare and Vitthalrao Bhimasha Khyade (2018). Quality Of The Cocoons And Silk Filament Obtained From The Larvae Of Silkworm, Bombyx Mori (L) (Race: Bivoltine Double Hybrid [(Po3 × Nd5) × (Csr4 × Csr2)] Fed With The Leaves Of Mulberry, Morus Alba (L) (M-5: Variety) Treated With Aqueous Solution Of Maple Syrup Belong To Acer Saccharum (L)', International Journal of Current Innovation Research, 4(3), pp. 1095-1100. www.journalijcir.com http://journalijcir.com/issues/quality-cocoons-and-silk-filament-obtained-larvae-silkworm-bombyx-mori-l-race-bivoltine
Sharad Ganpat Jagtap (2014). Influence of plant extractives on the silkworm, Bombyx mori (L). Ph. D. Thesis, SHRI JAGDISH PRASAD JHABARMAL TIBREWALA UNIVERSITY, VIDYANAGARI, JHUNJHUNU, RAJASTHAN – 333001 India.
DOI: https://doi.org/10.37591/jomcct.v10i3.2485
Refbacks
- There are currently no refbacks.