Method development and validation of RP-HPLC for simultaneous estimation of etoricoxib and Thiocolchicosidein tablet dosage forms

G. Suresh Kumar, D. Varun, P. Venkatesh, Narmada Valla Keerthi, Rajashakar. V

Abstract


Objective: A New approach became set up for simultaneous estimation of Etoricoxib and Thiocolchicoside with the aid of RP-HPLC approach.

Methods: Chromatographic separations were carried using Agilent XDB C18, 150 x 4.6 mm, 5m, column with a mobile phase composition of Sodium Phospahte buffer 3.5 pH and Acetonitrile (20:80) Were delivered at a glide price of 1ml/min and the detection changed into done the usage of waters HPLC automobile sampler, separation module 2695 HPLC gadget with PDA detector at wavelength 260 nm.

Results:  The maintenance time for Etoricoxib and Thiocolchicoside were 2.202 and 3.26 moment separately. The connection coefficient esteems in linearity were viewed as 0.999 and fixation range 80-400 g/ml for Etoricoxib and 20-100 g/ml for Thiocolchicoside individually. For exactness the all-out recuperation was viewed as 100.43 % and 100.28 % for Etoricoxib and Thiocolchicoside. LOD and LOQ for Etoricoxib 3.00 and 10.02. LOD and LOQ for Thiocolchicoside 3.02 and 10.07.

Conclusion: The consequences of study showed that the proposed RPHPLC technique is a straightforward, exact, exact, tough, powerful, quick and reproducible, which might be valuable for the standard assessment of Etoricoxib and Thiocolchicoside in tablet measurements structure.


Keywords


Etoricoxib, Thiocolchicoside, RP‐HPLC, Simultaneous estimation, Tablet dosage form

References


Overington JP, Al-Lazikani B, Hopkins AL: How many drug targets are there? Nat Rev Drug Discov. 2006 Dec;5(12):993-6. [PubMed:17139284]

Imming P, Sinning C, Meyer A: Drugs, their targets and the nature and number of drug targets. Nat Rev Drug Discov. 2006 Oct;5(10):821-34. [PubMed:17016423]

Capone ML, Tacconelli S, Di Francesco L, Sacchetti A, Sciulli MG, Patrignani P: Pharmacodynamic of cyclooxygenase inhibitors in humans. Prostaglandins Other Lipid Mediat. 2007 Jan;82(1-4):85-94. Epub 2006 Jul 3. [PubMed:17164136]

FitzGerald GA: COX-2 in play at the AHA and the FDA. Trends Pharmacol Sci. 2007 Jul;28(7):303-7. Epub 2007 Jun 18. [PubMed:17573128]

To compare the efficacy and safety of fixed dose combination of thiocolchicoside and aceclofenac versus chlorzoxazone, aceclofenac and paracetamol in patients with acute lower backache associated with muscle spasm [Link]

Thiocolchicoside as muscle relaxant: a review

Mascia MP, Bachis E, Obili N, Maciocco E, Cocco GA, Sechi GP, Biggio G: Thiocolchicoside inhibits the activity of various subtypes of recombinant GABA(A) receptors expressed in Xenopus laevis oocytes. Eur J Pharmacol. 2007 Mar 8;558(1-3):37-42. Epub 2006 Dec 12. (PubMed ID 17234181)

Goyal N, Bhandari A, Jain S, Patel R. Method development and validation of Etoricoxib and Thiocolchicoside in combined pharmaceutical solid dosage form by RP-HPLC method. International Journal of Pharmaceutical Studies and Research. 2011;2(1):106-109.

Kumar SS, Natraj D, Khan A, Kumar BK, Rao JV. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Etoricoxib and Thiocolchicoside in pharmaceutical dosage forms. International journal of research in pharmacy and chemistry2011;1(3):649-653.

Wankhede SB, Zambare SS, Dixit NR, Chitlange SS. RP-HPLC Method for Simultaneous Estimation of Thiocolchicoside and Ketoprofen in Combined Dosage Form. Scholars Research Library. 2010;2(3):315- 320. 20.

Dhaneshwar SR, Raut KO, BhusariVK. Validated HPLC method for simultaneous estimation of paracetamol, aceclofenac and thiocolchicoside in bulk drug and formulation. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2011;2(2): 435-445.

Kumar S, Joshi A, Thakur RS, Pathak AK, Shah K. simultaneous estimation of Etoricoxib and Thiocolchicoside by RP-HPLC method in combined dosage forms. ActaPoloniae Pharmaceutica n Drug Research. 2011;68 (6):839-845.

Acharjya SK, Mallick P, Panda P, Mathrusri MA. Spectrophotometric methods for the determination of thiocolchicoside in bulk and pharmaceutical dosage forms. J Pharm Educ Res. 2010;1(1):51-57.

Joshi RR and Gupta KR. Simultaneous UV- Spectrophotometric determination of Thiocolchicoside and Diclofenac in Pharmaceutical formulation. Pelagia Research Library. 2010;1(2):44-51.




DOI: https://doi.org/10.37591/jomcct.v12i3.3238

Refbacks

  • There are currently no refbacks.