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DNA Barcoding and Sequence Analysis of Annona reticulata Linn.

V. S. Sangeetha, Adheena Elza Johns, Beena Lawrence

Abstract


Abstract

DNA barcoding is the use of short DNA sequences (~650 bp) of the standard segment of genome for large scale species identification. The Consortium for the Barcode of Life (CBOL) plant-working group recommended 2-locus combination of rbcL and matK as the standard plant barcode. These two regions of chloroplast DNA were chosen due to efficient recovery of quality sequences and high levels of species discrimination. In this study, seven samples of Annona reticulata collected from different geographical locations were sequenced and analyzed for matK and rbcL genes by using bioinformatics tools to establish a molecular monogram. A.reticulata is the most medicinally important species of the Annonaceae family. The chloroplast matK marker was more informative than the rpoC1 chloroplast DNA markers. Phylogenetic trees were constructed on the basis of matK and rbcL gene sequences, and the results suggested that matK alone or in combination with rbcL can be used for determining the levels of genetic variation and for barcoding.

 Keywords: Barcoding, plants, matK, rbcL, PCR

Cite this Article

Sangeetha VS, Johns AE, Lawrence B. DNA Barcoding and Sequence Analysis of Annona reticulata Linn. Research & Reviews: Journal of Herbal Science. 2018; 7(3): 24–32p.



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