Open Access Open Access  Restricted Access Subscription or Fee Access

Antiurolithiatic Bioactives from Rotula aquatica Lour

Prashanthi P., Anitha S., Shashidhara S.


Urolithiasis is a complex process of kidney stone formation involves various stages of nucleation and mineralization. Plants are used for treatment of urolithiasis from early times. Thus the study was evolved around the plant Rotula aquatica Lour. used as “Paashanabhedha”. The present study reveals the bioactivity guided isolation of anti-urolithiatic compounds from successive solvent extracts of the roots of Rotula aquatica Lour. The isolated compounds showed significant in-vitro antiurolithiatic activity compared to Cystone®. The active aqueous extract was partitioned by column chromatography. The active 4th fraction was re-chromatographed to 12 sub-fractions. The active 7th and 8th sub-fractions were combined and subjected to prep-TLC. Two compounds, RA-01 and RA-02 had significant activity % inhibition of 45.21, 46.58, 49.74% and 46.53, 49.57, 54.37% at 0, 60, 120 minutes respectively. The results suggest the dose depended inhibition of crystal formation. Compounds were characterized and structure was elucidated as class of phenolic compounds: gallic acid and p-coumaric acid by spectral studies. The probable mechanisms include hydrolysis of calcium oxalate and calcium phosphate in presence of –OH of the isolated compounds. Thus  the study proves traditional use of Rotula aquatica Lour. in treatment of kidney stones.


Keywords: Rotula aquatica Lour., anti-urolithiatic, in-vitro bioactivity guided isolation, characterization, gallic acid, p-coumaric acid

Cite this Article

Prashanthi P. Antiurolithiatic Bioactives from Rotula Aquatica Lour. Research & Reviews: Journal of Herbal Science. 2019; 7(3): 1–10p.


Rotula aquatica Lour., anti-urolithiatic, in-vitro bioactivity guided isolation, characterization, gallic acid, p-coumaric acid

Full Text:



Monika G, Seema B, Sikka SK (2011). Role of Urinary Inhibitors and Promoters in Calcium oxalate Crystallisation. Int. J of Res. in Pharmacy and Chem., 1: 793-798.

Satish BNK, Kamal GK, Srinivasa V, Syed B (2012). A Review on Urolithiasis. Int. J Universal Pharmacy and Life Sci., 2: 269-280.

Joseph KC, Parekh BP, Joshi MJ (2005). Inhibition of growth of urinary type calcium hydrogen phosphate dihydrate crystals by tartaric acid and tamarind. Curr. Sci., 2005; 88: 1232-1238.

Ruth AH, Carol MP, Charlotte PP (2009). Pathophysiology: Concepts of Altered Health States. Chapter 3: Disorders of Renal system, pp 799.

Anand T, Vivek S, Vikas L, Ashish B, Deepti B, Sonali N (2012). An Overview on potent Indigenous Herbs for Urinary Tract Infirmity: Urolithiasis. Asian J Pharm. and Clinical Res., 5: 7-12.

Veronika B., Saeed RK (2009). Herbal Medicines in the Management of Urolithiasis: Alternative or Complementary? Planta Medica., 75: 1095–1103.

Rahul DY (2011), Herbal plants used in the treatment of Urolithiasis: A Review. Indian J Pharma Sci.- Review & Research., 2: 1412-1420.

Sheetal V, Singh SP (2010). Current and future status of herbal medicines. Veternary World., 1: 347-350.

Mukerjee T, Bhalla N, Singh G Jain HC (1984). Herbal drugs for urinary stones. Indian Drugs., 224-228.

Surendra KP, Kartik CP, Papiya MM, Dinakar S (2011). Establishing the Principle of Herbal Therapy for Antiurolithiatic Activity: A Review. J of Pharmacology and Toxi., 6: 321-332.

Anonymous (1972). Wealth of India, Raw materials. New Delhi, India: CSIR, 9: pp 80.

Warrier PK, Nambiar VPK, Ramankutty C (1996). Indian Medicinal Plants: A Compendium of 500 species. Chennai: Orient Longman Limited, pp 5.

Atta UR, Gary WC, Barry A (2005). Hyphenated Spectroscopic Methods. Frontiers in Drug Design and Discovery. Springer, 1: 118-119.

Havagiray RC, Shashi A, Jain SK, Monika S (2010). Herbal Treatment for Urinary stones. Int. Journal of Pharma. Sci. Res., 1: 24-31.

Hostettmann K, Terreaux C (2000). Search for new lead compounds from higher plants. Chimia., 54: 652-657.

Prashanthi P, Anitha S, Shashidhara S (2015). Effect of Rotula aquatica Lour. on Experimental Kidney Stones. Int. J of Pharmacognosy and Phytochemical Res., 7(6); 1142-1146.

Vivek VB, Vasanth kumar PK, Marina GD (2010). Effect of Phenolic compounds from Bergenia ciliate (Haw.) Sternb leaves on Experimental Kidney stones. Ancient Sci. of Life., 30: 14-17

Goyal PK, Mittal A, Kumar R. Evaluation of Tinospora cordifolia for Anti-urolithiatic Potential (2010). J Pharm. Biomed. Sci., 9: 1-7.

Chaudhary A, Singla SK, Tandon C (2010). In-vitro Evaluation of Terminalia arjuna on Calcium Phosphate and Calcium Oxalate Crystallization. Indian J Pharm.Sci., 72: 340–345.

Jethi RK, Bindhia D, Sahata RS, Gupta M, Sofat IB (1983). Effect of aqueous extract of an Ayurvedic compound preparation on mineralisation and demineralisation. Indian J Med. Res., 78: 422-425.

Jayaram DB, Anil NA, Subhash CM, Belsarea DP, Subodh CP (2010). Isolation and Characterization of hydrolysable tannins from leaf of Terminalia belerica Roxb. J Pharm Res., 3: 2410-2414.


  • There are currently no refbacks.

Copyright (c) 2019 Research & Reviews: Journal of Herbal Science