Molecular Diversity of Lampito mauritii and Perionyx excavatus Across North Eastern Ghats of Odisha Using RAPD Markers

Abstract

RAPD-PCR technique using 15 RAPD random primers was used to analyze genetic variation of Lampito mauritti and Perionyx excavatus adult worms collected from the agroclimatic zone of North Eastern Ghats of Odisha which includes four different districts. Studies on molecular characterization of L. mauritti and P. excavatus from different geographical locations indicated variation in the molecular profiling. Analysis of genetic diversity was performed using DNA marker systems with RAPD to define genetic relationships among 24 isolates of earthworm. RAPD-PCR data showed significant differentiation among population. The two species analysed in the present study showed high effective population sizes so that within each sampling site a high genetic diversity was observed. RAPD markers were found to be the most effective among the various marker systems used in the present days for the genetic characterization of earthworm individuals.

Keywords: Lampito mauritti, Perionyx excavatus, RAPD-PCR, genetic variation

Cite this Article

Sankar AS, Patnaik A, Agarwal P. Molecular Diversity of Lampito mauritii & Perionyx excavatus across North Eastern Ghats of Odisha Using RAPD Markers. Research & Reviews: A Journal of Life Sciences. 2018; 8(2): 33–41p