Amplification of 16s rRNA Gene of Brucella melitensis Causing Brucellosis Isolated from the Aborted Fetes of Goat

Abstract

ABSTRACT
Brucella melitensis is zoonosis caused by facultative intracellular bacteria of genus Brucella, which are widely distributed in both human and animals, especially in developing world. The diagnosis of goat brucellosis requires isolation of the bacteria from stomach contain aborted fetes of goat. However cultural sampling sensitivity often low, depending on the disease stage, Brucella species, culture medium, quantity of circulating bacteria and blood culture technique employed. The development of PCR has offered new dimension in the diagnosis deference microorganisms, which is possible in just few hours. Over the past decade, there has been major advancement all aspect of molecular diagnostic with regard to goat brucellosis. PCR based test providing to be fast and more sensitive than traditional methods. However sensitivity and specificity of the PCR for Brucella vary between laboratory and no standardization of sample preparation, target gene and detection method have been stabilized. There for in this study all the important aspect of the PCR for Brucella 16s rRNA gene detected and its diagnosis of Brucella a 1412 bp band corresponding to 16s rRNA gene of Brucella melitensis 16M strain was amplified.16s rRNA is conserved gene in Brucella spp. It is concluded that 16s rRNA gene could be used as a diagnostic tool and may prove as a candidate gene for development of subcellular vaccine again Brucellosis in goats.

Keywords: Brucella melitensis, PCR, gel electrophoresis, 16s rRNA, agglutination, DNA estimation, primers, oligonucleotides, NSS

Cite this Article
Akhand Pratap Singh, Jagriti Sharma. Amplification of 16s rRNA Gene of Brucella melitensis Causing Brucellosis Isolated from the Aborted Fetes of Goat. Research & Reviews: Journal of Veterinary Science and Technology. 2020; 9(1): 8–12p.