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Influence of Methanolic Extractives of Prepupal Stages of Black Soldier, Hermetia Illucens L. (Diptera: Stratiomyidae) on the Biochemical Parameters of Serum and Liver in the DMBA Induced Toxicity in Norwegian Rat, Rattus Norvegicus (L)

Vitthalrao Bhimasha Khyade


The larvae of Black soldier fly (BSF), Hermetia illucens L., are reducing the environmental pollution through utilization of organic waste for
their development on organic wastes and converting the organic waste into protein and fat rich biomass. Health promoting potentials of black
soldier fly derived proteins as ingredient of diet appears to be crucial need of modern days. In present attempt, forty Norwegian Rats were
utilized as experimental animals. The individuals of experimental animals were divided into four groups. Each group was consisted of ten individuals
of the experimental animals. The groups of the experimental animals include: Untreated control group; DMBA treated group; Methanolic Extractives of
Prepupal Stages of Black Soldier Fly (MEPSBSF) Treated group and group treated with DMBA and Methanolic Extractives of Prepupal Stages of Black Soldier
Fly (BSF) (DMBA + MEPSBSF). Experimental animals sacrificed at the end of experiment. The enzyme superoxide-dismutase (serves to breakdown the oxygen
molecules of potentially harmful category); the enzyme glutathione-peroxidase (serving for reducing the hydrogen peroxide to water); the nitric oxide
(compound responsible to cause widening of blood-vessels and stimulating hormones like insulin and growth hormone in human being); the enzyme myeloperoxidase
(catalysing the process of formation of a number of reactive oxidant species); enzyme Asparate-aminotransferase and the enzyme Alanine-aminotransferase are the
parameters considered for bioassay from the serum. Histopathological study on liver tissue was evaluated. The level of activity of enzyme Asparate-aminotransferase;
the enzyme Alanine-aminotransferase; the level of nitric oxide; myloperoxide in serum and the level of nitric oxide; myloperoxide in the liver tissue appear
to be significantly higher in carcinogen induced toxicity. The level of activity of enzyme Asparate-aminotransferase; the enzyme Alanine-aminotransferase;
the level of nitric oxide; myloperoxide in serum in the group of experimental animals in the group: “MEPSBSF + DMBA” in comparison with the group of
experimental animals in “untreated control”. The histological preparation of the liver tissue of the group of experimental animals in the group:
“MEPSBSF + DMBA” reported less sinusoidal dilation; vacuolization in the cytoplasm of the liver cells (hepatocytes). There was inflammation around
the central vein and in portal region in the group of experimental animals in the group: “MEPSBSF + DMBA”. Methanolic extractives of prepupal stages
of black soldier fly (MEPSBSF) was reported to reduce the stress exerted through oxidation (oxidative stress). This may be through induction of
mechanism of antioxidation process of the contents of Methanolic extractives of prepupal stages of black soldier fly (MEPSBSF). The present attempt
on utilization of Methanolic Extractives of Prepupal Stages of Black Soldier (MEPSBSF) is reporting protective influence against the carcinogenic
compounds like DMBA.


DMBA, Oxidative Stress, Antioxidants, Histopathology, Hepatotoxicity, MEPSBSF

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Rivenbark, A.G. and Coleman, W.B. (2014). An Introduction to the Conspicuous and Distinguishing Characteristics of Neoplasms. in Pathobiology of Human Disease, 2014A Dynamic Encyclopedia of Disease Mechanisms 2014, Pages 349-366. https://www.

Miyata M; Furukawa M; Takahashi K; et al. Mechanism of 7, 12-Dimethylbenz[a]anthracene-Induced Immunotoxicity: Role of Metabolic Activation at the Target Organ. Jpn J Pharmacol. 2001; 86(3): 302–309. doi:10.1254/jjp.86.302.

Sung YM; He G; Fischer, SM, et al. Lack of Expression of the EP2 but not EP3 Receptor for Prostaglandin E2 Results in Suppression of Skin Tumor Development. Cancer Res. 2005; 65(20): 9304–9311. doi:10.1158/0008-5472. can-05-1015.

FAO (2020). The State of World Fisheries and Aquaculture 2020 [online]. Available from

Herrero, M.; Wirsenius, S.; Henderson, B.; et al. Livestock and the environment: What have we learned in the past decade? Annu. Rev. Environ. Resour. 2015; 40: 177–202.

Herrero, M.; Henderson, B.; Havlík, P.; et al. Greenhouse gas mitigation potentials in the livestock sector. Nat. Clim. Chang. 2016; 6: 452–461.

Kanianska, R. (2016). Agriculture and its impact on land-use, environment, and ecosystem services. Landscape Ecology—The Influences of Land Use and Anthropogenic Impacts of Landscape Creation. Kanianska, R., Ed.; Slovakia. 2016, pp. 1–26. https://www.intechopen.



Ziolkowska, J. Economic and environmental costs of agricultural food losses and waste in the US. ETP Int. J. Food Eng. 2017; 3 (2): 140–145.

Liceaga, A.M. Approaches for utilizing insect protein for human consumption: Effect of enzymatic hydrolysis on protein quality and functionality. Ann. Entomol. Soc. Am. 2019; 112 (6): 529–532.

Anaya, J.-M.; Shoenfeld, Y.; Rojas-Villarraga, A.; et al. Autoimmunity: From Bench to Bedside. Bogota, Colombia: El Rosario University Press; 2013. ISBN 9587383664.

Omotoso, O.T. Nutritional quality, functional properties and anti-nutrient compositions of the larva of Cirina forda (Westwood) (Lepidoptera: Saturniidae). J. Zhejiang Univ. Sci. B. 2006; 7 (1): 51–55.

Van Huis, A. Potential of insects as food and feed in assuring food security. Annu. Rev. Entomol. 2013; 58: 563–583.

Borremans, A.; Bußler, S.; Sagu, S.T.; et al. Effect of Blanching Plus Fermentation on Selected Functional Properties of Mealworm (Tenebrio molitor) Powders. Foods. 2020; 9 (7): 917.

Ovissipour, M.; Rasco, B.; Shiroodi, S.G.; et al. Antioxidant activity of protein hydrolysates from whole anchovy sprat (Clupeonella engrauliformis) prepared using endogenous enzymes and commercial proteases. J. Sci. Food Agric. 2013; 93 (7): 1718–1726.

Hall, F.G.; Jones, O.G.; O’Haire, M.E.; Liceaga, A.M. Functional properties of tropical banded cricket (Gryllodes sigillatus) protein hydrolysates. Food Chem. 2017; 224: 414–422.

Purschke, B.; Meinlschmidt, P.; Horn, C.; et al. Improvement of techno-functional properties of edible insect protein from migratory locust by enzymatic hydrolysis. Eur. Food Res. Technol. 2018; 244: 999–1013.

Tang, Y.; Debnath, T.; Choi, E.-J.; et al. Changes in the amino acid profiles and free radical scavenging activities of Tenebrio molitor larvae following enzymatic hydrolysis. PLoS ONE. 2018; 13 (5): e0196218.

Caligiani, A.; Marseglia, A.; Leni, G.; et al. Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin. Food Res. Int. 2018; 105: 812–820.

Firmansyah, M.; Abduh, M.Y. Production of protein hydrolysate containing antioxidant activity from Hermetia illucens. Heliyon. 2019; 5(6): e02005.

Mintah, B.K.; He, R.; Dabbour, M.; et al. Characterization of edible soldier fly protein and hydrolysate altered by multiple-frequency ultrasound: Structural, physical, and functional attributes. Process Biochem. 2020; 95: 157–165.

Zhu, D.; Huang, X.; Tu, F.; et al. Preparation, antioxidant activity evaluation, and identification of antioxidant peptide from black soldier fly (Hermetia illucens L.) larvae. J. Food Biochem. 2020; 44(5): e13186.

Vitthalrao B. Khyade. Rearing the Black Soldier Fly, Hermetia illucens (Linnaeus) (Diptera: Stratiomyidae) in local environmental conditions of Baramati (India). Uttar Pradesh Journal of Zoology. 2021; 42 (5): 64–72.

Vitthalrao B. Khyade. Larval Instars of Black Soldier Fly for Converting Food Waste into Livestock Feed. EC Veterinary Science. 2021; 6.6: 50-53


V. B. Khyade and A. B. Tamhane. Utilization Of the Methanol Maceratives of Prepupal Stages of The Black Soldier Fly, HERMETIA ILLUCENS L. (DIPTERA: STRATIOMYIDAE) For Inhibition of Bacterial Growth. International Journal Of Researches In Biosciences, Agriculture and Technology. I J R B A T. 2021; Issue (Special-17); 485-499. e-ISSN 2347–517X.

Pedro CD, Mehab H, Guillermo FDJ, Selin Y. (2014). Development of a Food waste Composting system using Black Soldier Fly larvae. 3rd annual R&D Student Competition-Greenovate NYS[Online]. Available at: 30th September 2018).

Briscoe AD, Chittka L. The evolution of color vision in insects. Annual Review of Entomology. 2001; 46 (1): 471–510.

Zhang J, Huang L, He J, et al. An Artificial Light Source Influences Mating and Oviposition of Black Soldier Flies, Hermetiaillucens. Journal of Insect Science. 2010; 10 (1): 202.

Sheppard C, Newton GL, Thompson SA, et al. A value added manure management system using the black soldier fly. Bioresource Technology. 1994; 50 (4):275–279.

Sheppard DC, Jeffery K, Tomberlin, et al. Rearing Methods for the Black Soldier Fly (Diptera: Stratiomyidae). J. Med. Entomol. 2002; 39 (4):695–698.

Shields EB. Raising earthworms for profit. 20th ed. Eagle River, WI: Shields Publications; 1982.

Tomberlin Jeffery, Craig Sheppard D. Factors Influencing Mating and Oviposition of Black Soldier Flies (Diptera: Stratiomyidae) in a Colony. Journal of Entomological Science. 2002; 37(4): 345-352.

Savonen, Carol (2005-05-13). Big maggots in your compost? They're soldier fly larvae. OSU Extension Service–Gardening. Oregon State University.

Spranghers, Thomas; Ottoboni, Matteo; Klootwijk, Cindy; et al. Nutritional composition of black soldier fly (Hermetiaillucens) prepupae reared on different organic waste substrates. Journal of the Science of Food and Agriculture. 2017; 97 (8): 2594–2600. doi:10.1002/jsfa.8081. ISSN 1097-0010. PMID 27734508.

Lalander, C.; Diener, S.; Zurbrügg, C.; et al. Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetiaillucens). Journal of Cleaner Production. 2019; 208: 211–219. doi:10.1016/j.jclepro.2018.10.017. ISSN 0959-6526.

Wang, Yu-Shiang; Shelomi, Matan. Review of Black Soldier Fly (Hermetiaillucens) as Animal Feed and Human Food. Foods. 2017; 6 (10): 91. doi:10.3390/foods6100091. ISSN 2304-8158. PMC 5664030. PMID 29057841.

Goldson SL, Neill MRM, Proffitt JR, et al. Seasonal variation in larval–instar head–capsule sizes of Argentine stem weevil, Listronotusbonariensis (kuschel) (Coleoptera;Curculionidae). Australian J Entomol. 2001; 40 (4): 371–375.

Mohammed SMG. Determination of Larval Instars of Black Cutworm Agrotisipsilon (Hufnagel) (Lepidoptera, Noctuidae). JordanJournal of Biological Sciences. 2011; 4(3):173-176.

Bonelli, Marco; Bruno, Daniele; Brilli, Matteo; et al. Black Soldier Fly Larvae Adapt to Different Food Substrates through Morphological and Functional Responses of the Midgut. International Journal of Molecular Sciences. 2020; 21(14): 4955. doi:10.3390/ijms21144955. ISSN 1422-0067. PMC 7404193. PMID 32668813.

Bruno, Daniele; Bonelli, Marco; De Filippis, Francesca; et al. The Intestinal Microbiota of Hermetiaillucens Larvae Is Affected by Diet and Shows a Diverse Composition in the Different Midgut Regions. Applied and Environmental Microbiology. 2019; 85 (2): e01864–18, /aem/85/2/AEM.01864–18. atom. doi:10.1128/AEM.01864-18. ISSN 0099-2240. PMC 6328772. PMID 30504212.

Holmes, L. A.; Vanlaerhoven, S. L.; Tomberlin, J. K. Substrate Effects on Pupation and Adult Emergence of Hermetiaillucens (Diptera: Stratiomyidae): Table 1. Environmental Entomology. 2013; 42(2): 370–374. doi:10.1603/en12255. PMID 23575028.

Choi W, Yun J, Chu J et al. Antibacterial effect of extracts of Hermetiaillucens (Diptera: Stratiomyidae) larvae against Gram-negative bacteria. 2012; 42 (5): 219–226.

Shah MD, Iqbal M. Diazinon-induced oxidative stress and renal dysfunction in rats. Food ChemToxicol. 2010; 48 (12): 3345–3353.

Yilmaz N, Yilmaz M, Altuntas I. Diazinon-induced brain tox¬icity and protection by vitamins E plus C. ToxicolInd Health. 2012; 28 (1): 51–57.

Teimouri F, Amirkabirian N, Esmaily H, et al. Alteration of hepatic cells glucose me¬tabolism as a non-cholinergic detoxication mechanism in coun¬teracting diazinon-induced oxidative stress. Hum ExpToxicol. 2006; 25(12):697–703.

Kori-Siakpere Ovie; Ikomi Robert Bemigho, Ogbe Martin Gbemi. Variations in alanine aminotransferase and aspartate aminotransferase activities in African catfish: Clarias gariepinus (Burchell, 1822) at different sublethal concentrations of potassium permanganate. Scientific Research and Essays. 2010; 5 (12): 1501–1505.

Yehia Mona, A. H.; Sabah, G. El-Banna; Aly B. Okab. Diazinon toxicity affects histophysiological and biochemical parameters in rabbits. Experimental and Toxicological Pathology. 2007; 59 (3–4): 215–225.

Mysorekar, V. V., Rao, S. G., Mahadev, K. C. Liver histology in patient on hemodialysis with chronic hepatitis C viral infection. Indian J Pathol Microbiol. 2008; 51(2): 182–185.

Norman T. J., Baily. Statistical methods in Biology. Cambridge: Cambridge University Press; 1995.

Taylor, W.H. Formol titration: An evaluation of its various modifications. Analyst 1957; 82(976): 488–498.

Salem R, Lewandowski R, Roberts C, et al. Use of Yttrium-90 glass microspheres (TheraSphere) for the treatment of unresectable hepatocellular carcinoma in patients with portal vein thrombosis. Journal of Vascular and Interventional Radiology. 2004; 15 (4): 335–345.

Hayyan M, Hashim MA, Al Nashef IM. Superoxide Ion: Generation and Chemical Implications. Chem. Rev. 2016; 116 (5): 3029–3085. doi:10.1021/acs.chemrev.5b00407. PMID 26875845.

Sanchez-Valle V., Chavez-Tapia N.C., Uribe M., et al. Role of oxidative stress and molecular changes in liver fibrosis: A review. Curr. Med. Chem. 2012; 19(28): 4850–4860. doi: 10.2174/092986712803341520.

Sakaguchi S., Takahashi S., Sasaki T., et al. Progression of alcoholic and non-alcoholic steatohepatitis: Common metabolic aspects of innate immune system and oxidative stress. Drug Metab. Pharmacokinet. 2011; 26:30–46. doi: 10.2133/dmpk.DMPK-10-RV-087.

Cichoz-Lach H., Michalak A. Oxidative stress as a crucial factor in liver diseases. World J. Gastroenterol. 2014; 20(25): 8082–8091. doi: 10.3748/wjg.v20.i25.8082.

Wu D., Cederbaum A.I. Oxidative stress and alcoholic liver disease. Semin Liver Dis. 2009; 29:141–154. doi: 10.1055/s-0029-1214370.

Li A.N., Li S., Zhang Y.J., et al. Resources and biological activities of natural polyphenols. Nutrients. 2014; 6(12): 6020–6047.

Giray B, Gurbay A, Hincal F. Cypermethrin-induced oxidative stress in rat brain and liver is prevented by vitamin E or allopurinol. Toxicol Lett. 2001; 118 (3): 139–146. doi: 10.1016/s0378-4274(00)00277-0.

Akturk, O., Demirin, H., Sutcu, R., et al. The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C. Cell Biol. Toxicol. 2006; 22(6):455–461.


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