Influence of Methanolic Extractives of Prepupal Stages of Black Soldier, Hermetia Illucens L. (Diptera: Stratiomyidae) on the Biochemical Parameters of Serum and Liver in the DMBA Induced Toxicity in Norwegian Rat, Rattus Norvegicus (L)

Abstract

The larvae of Black soldier fly (BSF), Hermetia illucens L., are reducing the environmental pollution through utilization of organic waste for
their development on organic wastes and converting the organic waste into protein and fat rich biomass. Health promoting potentials of black
soldier fly derived proteins as ingredient of diet appears to be crucial need of modern days. In present attempt, forty Norwegian Rats were
utilized as experimental animals. The individuals of experimental animals were divided into four groups. Each group was consisted of ten individuals
of the experimental animals. The groups of the experimental animals include: Untreated control group; DMBA treated group; Methanolic Extractives of
Prepupal Stages of Black Soldier Fly (MEPSBSF) Treated group and group treated with DMBA and Methanolic Extractives of Prepupal Stages of Black Soldier
Fly (BSF) (DMBA + MEPSBSF). Experimental animals sacrificed at the end of experiment. The enzyme superoxide-dismutase (serves to breakdown the oxygen
molecules of potentially harmful category); the enzyme glutathione-peroxidase (serving for reducing the hydrogen peroxide to water); the nitric oxide
(compound responsible to cause widening of blood-vessels and stimulating hormones like insulin and growth hormone in human being); the enzyme myeloperoxidase
(catalysing the process of formation of a number of reactive oxidant species); enzyme Asparate-aminotransferase and the enzyme Alanine-aminotransferase are the
parameters considered for bioassay from the serum. Histopathological study on liver tissue was evaluated. The level of activity of enzyme Asparate-aminotransferase;
the enzyme Alanine-aminotransferase; the level of nitric oxide; myloperoxide in serum and the level of nitric oxide; myloperoxide in the liver tissue appear
to be significantly higher in carcinogen induced toxicity. The level of activity of enzyme Asparate-aminotransferase; the enzyme Alanine-aminotransferase;
the level of nitric oxide; myloperoxide in serum in the group of experimental animals in the group: “MEPSBSF + DMBA” in comparison with the group of
experimental animals in “untreated control”. The histological preparation of the liver tissue of the group of experimental animals in the group:
“MEPSBSF + DMBA” reported less sinusoidal dilation; vacuolization in the cytoplasm of the liver cells (hepatocytes). There was inflammation around
the central vein and in portal region in the group of experimental animals in the group: “MEPSBSF + DMBA”. Methanolic extractives of prepupal stages
of black soldier fly (MEPSBSF) was reported to reduce the stress exerted through oxidation (oxidative stress). This may be through induction of
mechanism of antioxidation process of the contents of Methanolic extractives of prepupal stages of black soldier fly (MEPSBSF). The present attempt
on utilization of Methanolic Extractives of Prepupal Stages of Black Soldier (MEPSBSF) is reporting protective influence against the carcinogenic
compounds like DMBA.