Method development and validation of RP-HPLC for simultaneous estimation of etoricoxib and Thiocolchicosidein tablet dosage forms

G. Suresh Kumar, D. Varun, P. Venkatesh, Narmada Valla Keerthi, Rajashakar. V


Objective: A New approach became set up for simultaneous estimation of Etoricoxib and Thiocolchicoside with the aid of RP-HPLC approach.

Methods: Chromatographic separations were carried using Agilent XDB C18, 150 x 4.6 mm, 5m, column with a mobile phase composition of Sodium Phospahte buffer 3.5 pH and Acetonitrile (20:80) Were delivered at a glide price of 1ml/min and the detection changed into done the usage of waters HPLC automobile sampler, separation module 2695 HPLC gadget with PDA detector at wavelength 260 nm.

Results:  The maintenance time for Etoricoxib and Thiocolchicoside were 2.202 and 3.26 moment separately. The connection coefficient esteems in linearity were viewed as 0.999 and fixation range 80-400 g/ml for Etoricoxib and 20-100 g/ml for Thiocolchicoside individually. For exactness the all-out recuperation was viewed as 100.43 % and 100.28 % for Etoricoxib and Thiocolchicoside. LOD and LOQ for Etoricoxib 3.00 and 10.02. LOD and LOQ for Thiocolchicoside 3.02 and 10.07.

Conclusion: The consequences of study showed that the proposed RPHPLC technique is a straightforward, exact, exact, tough, powerful, quick and reproducible, which might be valuable for the standard assessment of Etoricoxib and Thiocolchicoside in tablet measurements structure.


Etoricoxib, Thiocolchicoside, RP‐HPLC, Simultaneous estimation, Tablet dosage form


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