Evaluation of Rhizobium Strains Inoculation on Growth, Yield, and Yield Components of Faba Bean in Debub Ari District, Southern Ethiopia

Atinafu Tunebo, Genanaw Tesema, Merdikios Malla


Production and productivity of faba beans in Ethiopia are reduced due to biotic and abiotic factors. Reduction of soil fertility is among the most important factors that reduce the productivity of faba beans, thus the trial was conducted to estimate Rhizobium strains inoculation effect on the production of faba bean in Debub Ari District, Southern Ethiopia. The trial was conducted on cropland in Debub Ari District during the 2016/2017- 2017/2018 cropping season from August-December. Eight treatments were used for the experiment namely; control, recommended NP, FB17, FB1035, EAL110, FB17+50kgha-1 DAP, FB1035+50kgha-1 DAP, and EAL110+50kgha-1 DAP; which was laid out in randomized complete block design by using improved faba bean variety Gebelcho. Faba bean seed was inoculated by Rhizobium strains properly on-farm and sown immediately after inoculation. Rhizobium strains inoculation had significantly affect plant height, pod per plant, and grain yield of faba bean; of which the highest plant height (118.77cm), pod per plant (22.17), and grain yield (2421.4 kg ha-1) were recorded in response to application of EAL110 with 50 kg ha-1 Di-ammonium phosphorus fertilizer, while the lowest plant height and grain yield was recorded from absolute control.  Inoculating faba bean seed with Rhizobium strain EAL110 with additional 50 kg ha-1 Di-ammonium phosphorus fertilizer increased yield up to 43.02% as compared to absolute control. Therefore, the application of rhizobium strains EAL110 with 50 kg ha-1 Di-ammonium phosphorus fertilizer was recommended for faba bean production in the study area, still further investigation should be important for determining the effectiveness of Rhizobium strains by isolating indigenous bacteria from the soil of the study area.


Nitrogen Fixation, Productivity, Rhizobium, Soil Fertility.


Abdel Latef, A. H. A., and Ahmad, P. (2015). Legumes and breeding under abiotic stress: an overview. Legumes under environmental stress: Yield, improvement and adaptations, 1-20.

Abera, T., Feyissa, D., and Abebe, Z. (2009). A review of Organic and Biological Soil Fertility Management integrated with NP on Crops Yield and Soil Fertility Improvement in high and mid altitude areas of Western Oromia, Ethiopia. In Improved Natural Resource Management for Food Security Poverty Reduction and Sustainable Development. 10th Annual Conference of Ethiopian Society of Soil Science March (pp. 26-27).

Abera, T., Semu, E., Debele, T., Wegary, D., and Kim, H. (2015). Determination soil rhizobium populations, intrinsic antibiotic resistance, nodulation and seed yield of faba bean and soybean in Western Ethiopia. World Journal of Agricultural Sciences, 11(5), 311-324.

Alemayehu, W. (2009). The effect of indigenous root-nodulating bacteria on nodulation and growth of Faba bean (Vicia faba) in the low-input agricultural systems of Tigray Highlands, Northern Ethiopia. Momona Ethiopian Journal of Science, 1(2).

Allito, B. B., Ewusi-Mensah, N., and Alemneh, A. A. (2014). Rhizobia strain and host-legume interaction effects on nitrogen fixation and yield of grain legume: A review.

Anteneh Argaw and Abere Mnalku. (2016). Nodulation, Yield, and Yield Traits of Faba bean (Vicia faba L.) Under Integrated Application of Vermicompost and Rhizobium leguminosarum bv Vicieae Inoculation at Haramaya, Eastern Ethiopia. In: "Proceedings of Annual Research Review Workshop", April 2016, Haramaya University, Ethiopia.

Anteneh Argaw. (2012). Characterization of symbiotic effectiveness of rhizobia nodulating Faba bean (Vicia faba L.) isolated from central Ethiopia. Research Journal of microbiology, 7(6), 280-296.

Araujo, J., Díaz-Alcántara, C. A., Velázquez, E., Urbano, B., and González-Andrés, F. (2015). Bradyrhizobium yuanmingense related strains form nitrogen-fixing symbiosis with Cajanus cajan L. in Dominican Republic and are efficient bio fertilizers to replace N fertilization. Scientia Horticulturae, 192, 421-428.

Batista, L., Irisarri, P., Rebuffo, M., Cuitiño, M. J., Sanjuán, J., and Monza, J. (2015). Nodulation competitiveness as a requisite for improved rhizobial inoculants of Trifolium pratense. Biology and Fertility of Soils, 51, 11-20.

Bejandi, T. K., Sharifii, R. S., Sedghi, M., and Namvar, A. (2012). Effects of plant density, Rhizobium inoculation and microelements on nodulation, chlorophyll content and yield of chickpea (Cicer arietinum L.). Annals of Biological Research, 3(2), 951-958.

Belay, Z., and Assefa, F. (2011). Symbiotic and phenotypic diversity of Rhizobium leguminosarum bv. viciae from Northern Gondar, Ethiopia. African Journal of Biotechnology, 10(21), 4372-4379.

Berhanu, D. (1980). Survey of studies conducted about soil resources appraisal and evaluation for rural development in Ethiopia.

Beyene, D. (1988). Biological Nitrogen Fixation Research on Grain Legumes in Ethiopia—an Overview. In Nitrogen Fixation by Legumes in Mediterranean Agriculture: Proceedings of a workshop on Biological Nitrogen Fixation on Mediterranean-type Agriculture, ICARDA, Syria, April 14–17, 1986 (pp. 73-78). Springer Netherlands.

Black, C. A. (1965). Methods of Soil Analysis Part one and two. American Society of Agronomy, Inc.; USA.

Blanco, R. A., Sicardi, M., and Frioni, L. (2010). Competition for nodule occupancy between introduced and native strains of Rhizobium leguminosarum biovar trifolii. Biology and fertility of soils, 46, 419-425.

Carter, J. M., Tieman, J. S., and Gibson, A. H. (1995). Competitiveness and persistence of strains of rhizobia for faba bean in acid and alkaline soils. Soil Biology and Biochemistry, 27(4-5), 617-623.

Charman, P. E. V., and Roper, M. M. (2007). Soil organic matter: In ‘Soils-their properties and management’.

CSA (Central Statistics Agency). 2016. The Federal Democratic Republic of Ethiopia Central Statistical agency agricultural sample survey (2015/2016) report on area and production of major crops (private peasant holding, Meher season). Volume I, Statistical Bulletin 532, Addis Ababa, Ethiopia, 2016.

CSA (Central Statistics Agency). 2018. The Federal Democratic Republic of Ethiopia Central Statistical Agency agricultural sample survey report on area and production of major crops (private peasant holdings, Meher season). Volume I, Statistical Bulletin 586, Addis Ababa Ethiopia.

Marx, E. S., Hart, J., and Stevens, R. G. (1999). Soil Test Interpretation Guide. Oregon State University. Soil Sci, 120, 1120-1322.

Emerson, W. W. (1991). Structural decline of soils, assessment and prevention. Soil Research, 29(6), 905-921.

Fageria, N. K., and Santos, A. D. (2008). Yield physiology of dry bean. Journal of plant nutrition, 31(6), 983-1004.

Getachew, A., Lakew, B., and Nelson, P. N. (2014). Cropping sequence and nitrogen fertilizer effects on the productivity and quality of malting barley and soil fertility in the Ethiopian highlands. Archives of Agronomy and Soil Science, 60(9), 1261-1275.

Getachew, A., Vanbeek, C., and Bird, M. I. (2014). Influence of integrated soil fertility management in wheat and tef productivity and soil chemical properties in the highland tropical environment. Journal of soil science and plant nutrition, 14(3), 532-545.

Gete, Z., Rashid, S., Abera, D., and Agegnehu, G. (2010). Fertilizer and soil fertility potential in Ethiopia.

Godara, A. S., Gupta, U. S., and Singh, R. A. V. I. N. D. R. A. (2012). Effect of integrated nutrient management on herbage, dry fodder yield and quality of oat (Avena sativa L.). Forage Research, 38(1), 59-61.

Habtemichial, H., K., Ram Singh, B., and Aune, J. B. (2007). Wheat response to N2 fixed by faba bean (Vicia faba L.) as affected by sulfur fertilization and rhizobial inoculation in semi‐arid Northern Ethiopia. Journal of Plant Nutrition and Soil Science, 170(3), 412-418.

Hasan, M., and Ismail, S. (2016). Evaluation of Rhizobium inoculation in combination with phosphorus and nitrogen fertilization on groundnut growth and yield. Journal of Agronomy, 15(3), 142-146.

Köpke, U., and Nemecek, T. (2010). Ecological services of faba bean. Field crops research, 115(3), 217-233.

Kudapa, H., Ramalingam, A., Nayakoti, S., Chen, X., Zhuang, W. J., Liang, X,... and Varshney, R. K. (2013). Functional genomics to study stress responses in crop legumes: progress and prospects. Functional Plant Biology, 40(12), 1221-1233.

Metson, A. J. (1961). Methods of chemical analysis for soil survey samples. Soil Bureau Bulletin No. 12, New Zealand Department of Scientific and Industrial Research. Government printer: Wellington, New Zealand, 168-175.

MoA (Ministry of Agriculture). (2011). Animal and plant health regulation directorate. Crop variety register. Issue No. 14. Addis Ababa, Ethiopia, 2011, pp. 71-73.

Mulas, D., Seco, V., Casquero, P. A., Velázquez, E., and González-Andrés, F. (2015). Inoculation with indigenous rhizobium strains increases yields of common bean (Phaseolus vulgaris L.) in northern Spain, although its efficiency is affected by the tillage system. Symbiosis, 67, 113-124.

Murphy, H. F. (1968). A report on the fertility status and other data on some soils of Ethiopia.

Proskina, L., Cerina, S., and Zeverte-Rivza, S. (2016). Faba beans as an alternative protein source for broiler chicken feed. In Economic Science for Rural Development Conference Proceedings (No. 41).

Reda, A., Abdel-Aziz, El-Sebai, T. N., El-Din, S. M. B., and Abo-Sedera, S. A. (2016). Field Application of Bio-Fertilizers Technology on Faba Bean Growth and Yield. RESEARCH Journal of Pharmaceutical Biological and Chemical Sciences, 7(3), 2481-2487.

Saidi, S., Ramirez-Bahena, M. H., Santillana, N., Zuniga, D., Álvarez-Martínez, E., Peix, A., and Velazquez, E. (2014). Rhizobium laguerreae sp. nov. nodulates Vicia faba on several continents. International Journal of Systematic and Evolutionary Microbiology, 64(Pt_1), 242-247.

Serraj, R., and Adu-Gyamfi, J. (2004). Role of symbiotic nitrogen fixation in the improvement of legume productivity under stressed environments. West African Journal of Applied Ecology, 6(1).

Siczek, A., and Lipiec, J. (2016). Impact of faba bean-seed rhizobial inoculation on microbial activity in the rhizosphere soil during growing season. International journal of molecular sciences, 17(5), 784.

Sofy, A. R., Attia, M. S., El-Monem, M. A., Sharaf, A. E. M. A., and El-Dougdoug, K. A. (2014). Bean yellow mosaic potyvirus potential on nodulation and N2-fixation of faba bean plants. Journal of New York Science, 7(9), 101-109.

Tekalign, T., Haque, I., and Aduayi, E. A. (1991). Soil, plant, water, fertilizer, animal manure & compost analysis manual.

Thies, J. E., Singleton, P. W., and Bohlool, B. B. (1991). Influence of the size of indigenous rhizobial populations on establishment and symbiotic performance of introduced rhizobia on field-grown legumes. Applied and environmental microbiology, 57(1), 19-28.

Upendra, S. M., Lenssen, A. W., and Barsotti, J. L. (2013). Dryland malt barley yield and quality affected by tillage, cropping sequence, and nitrogen fertilization. Agronomy Journal, 105(2), 329-340.

Woldekiros, B. (2018). Response of faba bean to Rhizobium Inoculation and potassium fertilizer rates at Alicho Wuriro highland, Southern Ethiopia. Int. J. R. Agri. and Fore, 5(5), 43-47.

Zahran, H. H. (1999). Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiology and molecular biology reviews, 63(4), 968-989.

Zarina, L., Alsina, I., and Vaivode, A. (2017). Effectiveness of Rhizobial Strains on the Faba Bean Development and Yield in Soddy Podzolic Soils. In Environment, Technologies, Resources. Proceedings of the International Scientific and Practical Conference (Vol. 1, pp. 305-308).

DOI: https://doi.org/10.37591/rrjob.v12i3.3804


  • There are currently no refbacks.

Copyright (c) 2024 Research & Reviews: Journal of Botany