In vitro Anthelmintic and Antibacterial Potentialities of Methanol Extract from the Leaf and Bark of Lawsonia inermis Linn. (Family: Lythraceae)

Abstract

Due to the availability of the genus Lawsonia in Bangladesh, the present study was aim to investigate the phytochemical screening, anthelmintic and antimicrobial potentialities of methanol extract from the leaf and bark of Lawsonia inermis L. The phytochemical screening was performed by chemical group tests for both the extract which indicate the presence of several chemical groups including steroids, alkaloids, flavanoids, tannins and saponins. The anthelmintic activity was performed by observing the time of paralysis and the time of death of earth worms Pheretima posthuma compared with the standard drug, Albendazole. The range of minimum to maximum time of paralysis and death of Albendazole at the conc. 100 µg/ml was 2 min 6 sec to 9 min 23 sec. whereas; the range of minimum to maximum time of paralysis and death of the fresh leaf juice was 2 min 36 sec to 3 min 27 sec. and the bark juice was 2 min 5 sec to 12 min 25 sec, respectively. The results confirm the presence of highly potent anthelmintic activity of both the extract. The antibacterial activity was performed by determining the zone of inhibition of living microorganisms compared with the standard drug, Ciprofloxacin. The range of minimum to maximum zone of inhibition of Ciprofloxacin was 15 to 25 mm whereas, the range of minimum to maximum zone of inhibition of the methanol extract of leaf and bark were 7 to 10 mm, respectively. These results showed very mild antimicrobial activity for both the extract.

Keywords: Lawsonia inermis L., Lythraceae, Anthelmintic activity, Antibacterial activity,Albendazole, Ciprofloxacin

Cite this Article
Sadia Afreen Chowdhury, In vitro Anthelmintic and Antibacterial Potentialities of the Methanol Extracts from the Leaf and Bark of Lawsonia inermis Linn. (Family: Lythraceae), Research and Reviews: Journal of Botany (RRJoB). 2015; 4(2): 1–5p.