In-vitro Plantlet Regeneration in Mint Genotypes Using Different Explants

Abstract

Abstract

Nine Genotypes and three explants were used for in vitro plantlet regeneration of mint in the Tissue Culture Laboratory of the Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, during March 2013 to January 2014. The growth regulators BAP and NAA were used @ 1 mg/l. Results revealed that the highest number of shoots were produced in shoot tip (1.94) at 55 days after inoculation (DAI), which initiated shoot within 20.09 days. Although, leaf showed an early (15.67days) response for root regeneration but the highest number of root was recorded in shoot tip (2.48) at 55 days after inoculation (DAI).  In case of genotypes, MP-1 (9.94 days) showed the early response for shoot initiation and MP-6(12.72 days) showed the early response for root initiation. The interaction of MP-1 and node produced the highest number of shoots (4.66) at 55 DAI; whereas, MP-6 with shoot tip showed early response (9.83 days) for root initiation. Finally, shoot tip and nodal segment was found to be the best for using as ex plants in mint and the genotype MP-1 and MP-6 performed better for plantlet regeneration in in vitro.

Keywords: Mint, plantlet regeneration, in vitro, explants, genotypes

Cite this Article

Tuly JA, Hoque MA. In-vitro Plantlet Regeneration in Mint Genotypes Using Different Explants. Research & Reviews : Journal of Crop Science and Technology. 2015; 4(2): 24–33p.