Sterilization Protocol Optimization for Geranium (Pelargonium graveolens L.) in vitro Propagation from Nodal Culture

Abstract

Abstract

The present study was initiated to optimize sterilization protocol for in vitro propagation protocol from nodal culture of geranium. Three (treatment 1, 2 and 3) surface sterilization treatments were tested. According to the results of the sterilization test, the percentage of contaminated magenta in treatment 1 and 2 was 65.71 and 60%, respectively, which was highly reduced to 11.42% when Kocide 101 was applied before treating explants with Ethanol and NaClO in treatment 3 (Table 1). Treatment 3 not only reduced the percentage of contamination, but also increased the survival rate of explants after the sterilization treatments. The results showed that the mortality rate of treatments 1 and 2 was 53.3% while that of treatment 3 was 23.8%. Treatment 3 surface sterilization (treatment of explants with 1.5% (w/v) fungicide (Kocide 101) mixed with one drop of soup solution for 1 min, 70% Ethanol for 1 min and with 1% NaClO for 3 min and again with 70% Ethanol for 1 min) was the best, and used throughout the experiment with minimum contamination and low percentages of dead nodal explants.

Keywords: Sterilization, KOCIDE, NaClO, propagation

Cite this Article

Tilahun Rabuma. Sterilization Protocol Optimization for Geranium (Pelargonium graveolens L.) in vitro Propagation from Nodal Culture. Research & Reviews: Journal of Crop Science and Technology (RRJoCST). 2015; 4(1): 34–38p.