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Determining the Antagonistic Potential of Lactobacilli Isolated from Commercial Curd Samples against the E. coli

Rahul Verma, Jagriti Sharma, Akhand Pratap Singh


Curd has always been an important source exhibiting a plethora of Lactobacilli. Lactobacilli are rod-shaped bacteria, capable of producing short-chain fatty acids, lactate, and acetate by carbohydrate fermentation process. A study was done to determine the antimicrobial activity of isolated and identified Lactobacilli bacteria from commercial curd samples against standard strains of E. coli. All the 10 samples were found have lactobacilli strains. The antagonistic activity of these strains was seen by preparing the serial dilution of Mcfarland standard # 1.0, serial dilution 1.0 was shown to produce maximum zone of inhibition followed by the suspensions of 1/10 and1/100.


Curd, lactobacilli, bacteria, antimicrobial activity, E. coli

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Madsen, K., Cornish, A., Soper, P., McKaigney, C., Jijon, H., Yachimec, C., Doyle, J., Jewell, L., De Simone, C., 2001. Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterology. 121, 580–591.

Isolauri, E.; Salminen, S.; Ouwehand, A.C. Probiotics 2004, Best Pract. Res. Cl. Ga. 18, 299–31.

Klaenhammer, T.R.; Kullen, M.J. Selection and design of probiotics. Int. J. Food Microbiol. 1999, 50, 45–57.

Vankerckhoven, V.; Huys, G.; Vancanneyt, M.; Vael, C.; Klare, I.; Romond, M.B., Goossens, H. Biosafety assessment of probiotics used for human consumption: recommendations from the EU-PROSAFE project. Trends Food Sci. Technol. 2008, 19, 102–114.

Bâati, L.L.; Fabre-Gea, C.; Auriol, D.; Blanc, P.J. (2000). Study of the cryotolerance of Lactobacillus acidophilus: Effect of culture and freezing conditions on the viability and cellular protein levels. International Journal of

Food Microbiology. 59 (3): 241–247. doi:10.1016/S0168–1605(00)00361–5. PMID 11020044.

Fijan, Sabina (2014). Microorganisms with Claimed Probiotic Properties: An Overview of Recent Literature. International Journal of Environmental Research and Public Health. 11 (5): 4745–4767. doi:10.3390/ijerph1 10504745. ISSN 1660–4601. PMC 4053917 . PMID 24859749.

Ghouri, Yezaz A; Richards, David M; Rahimi, Erik F; Krill, Joseph T.(2014). Jelinek, Katherine A; DuPont, Andrew W Systematic review of randomized controlled trials of probiotics, prebiotics, and synbiotics in inflammatory bowel disease. Clin Exp Gastroenterol. 7: 473–487. doi:10.2147/CEG.S275 30. PMC 4266241 . PMID 25525379.

Behare P, Kumar H, Mandal S.(2016). Yogurt: yogurt-based products. Encyclopedia of Food and Health. p. 625–631.


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