Detection of Two Different Morphotypes of Mitochondria in the Live Hemocytes of the Sand Lobster (Thenus orientalis)

Abstract

Abstract

In this in vitro study, presence of mitochondria in live blood cells or hemocytes in monolayer of the sand lobster, Thenus orientalis has been detected using rhodamine 123 staining. In freshly made hemocyte monolayers (after 40 minutes of hemocyte monolayer preparation), many hemocytes showed mitochondria with fine, filamentous (elongated) morphology in their cytoplasm. These structures were highly dynamic and were actively moving around in these cells. However, in aged hemocyte monolayers (>1.5 hours old), the terminal ends of the elongated mitochondria were fused to form a donut- or ring-shaped structure. The presence of two different mitochondrial morphotypes have been microscopically observed and the possible significance of occurrence of these donut- or ring-shaped mitochondria is discussed in this study.

Keywords: Hemocytes, rhodamine 123, filamentous and donut-shaped mitochondria, aged hemocyte monolayer

Cite this Article

Vasudevan Vaishnavi, Periasamy Mullainadhan, Detection of Two Different Morphotypes of Mitochondria in the Live Hemocytes of the Sand Lobster (Thenus orientalis). Research & Reviews: A Journal of Life Sciences. 2019; 9(1): 10–14p.