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An Improved Method for the Isolation of High Quality Genomic DNA from Axenic Cultures of Filamentous Blue-green algae (Cyanobacteria), Nostoc and Anabaena

Swetha Thilak T., P. V. Madhusoodanan, R. Prakashkumar, N. S. Pradeep


Blue-green algae are one of the important organisms having different ecological and economic important properties and are among one of the least explored one. The major difficulty is met with their exact identity; this could be tackled to an extent with the use of molecular techniques. Isolation of high quality DNA is essential for molecular biology applications. In the present study an efficient protocol for the extraction of genomic DNA from cultured samples of Nostoc and Anabaena collected from different districts of Kerala is developed. The DNA was found suitable for PCR amplification. The A260/280 of the isolated DNA was found to be 1.5–1.8 indicating their purity.


Keywords: Isolation, DNA, blue-green algae, PCR amplification, axenic culture

Cite this Article

Swetha Thilak T. P.V. Madhusoodanan R. Prakash kumar N.S. Pradeep. An Improved Method for the Isolation of High Quality Genomic DNA from Axenic Cultures of Filamentous Blue-green algae (Cyanobacteria), Nostoc and Anabaena. Research & Reviews: A Journal of Life Sciences. 2019; 9(2): 32–38p.


Isolation; DNA; Blue-green algae; PCR amplification; Axenic culture

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