Green synthesis of zinc oxide and copper oxide nanoparticles and their effect against wilt fungus, Fusarium udum.

Geeta Rautela, Rose Rizvi

Abstract


Nowadays nanoparticles (NPs) come out as powerful tools to enhance crop production and protection. Biosynthesis of NPs has a vital role and an alternate to chemical and physical methods because it is simple, easy, eco-friendly, and free from chemical hazards. Zinc oxide (ZnO) and copper oxide (CuO) NPs were synthesized from leaves extract of Lantana camara (L.) and Nerium oleander (L.) and characterized by using several techniques such as transmission electron microscope (TEM), scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX), UV-visible spectroscopy, and fourier transform infrared spectroscopy (FTIR). ZnO and CuO NPs were synthesized from the leaves extract of Lantana camara (L.) and Nerium oleander (L.) respectively. ZnO NPs and CuO NPs exhibited an inhibitory effect against the wilt fungi, Fusarium udum. Different concentrations of ZnO NPs (50µg/mL, 100µg/mL, and 200µg/mL) and CuO NPs (20 ppm, 50 ppm, and 100 ppm) were tested against the wilt fungi. Both the nanoparticles, ZnO NPs, and CuO NPs inhibited the growth of Fusarium udum but in dose dose-dependent manner. Results revealed that the growth of Fusarium udum was inhibited by various concentrations of ZnO and CuO NPs but significant reduction occurred at 100 µg/mL in ZnO NPs and 100 ppm in CuO NPs respectively. Overall, ZnO and CuO NPs positively reduced the growth of Fusarium udum. Therefore, the implementation of biosynthesized ZnO and CuO NPs could be considered eco-friendly and effective agents for crop protection as compared to chemical fungicides.

References


Siddiqi KS, Husen A. Properties of zinc oxide nanoparticles and their activity against microbes. Nanoscale Res. Lett. 2018;13 (1), 1-13.

Duran N, Durán M, de Jesus MB, Seabra, AB, Fávaro WJ, Nakazato. G. Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity. Nanomed. 12 (3), 2016;789–799.

Zulfiqar F, Navarro M, Ashraf M, N.A., Akram NA, Munné-Bosch S. Nanofertilizer use for sustainable agriculture. Advantages and limitations. Plant Sci. 2019;289:110270.

Tiwari V, Mishra N, Gadani K, Solanki PS, Shah NA, Tiwari M. Mechanism of anti-bacterial activity of zinc oxide nanoparticle against carbapenem-resistant Acinetobacter baumannii. Front. Microbiol. 9 (6), 2018;1218.

Mirzaei H, Darroudi M., Zinc oxide nanoparticles. Biological synthesis and biomedical applications. Ceram. Int. 2017;43(1), 907-914

Khan ST, Musarrat J, Al-Khedhairy CA. Countering drug resistance, infectious diseases, and sepsis using metal and metal oxides nanoparticles. Current status. Colloids Surf. B. 146, 2016;70–83.

Maqusood A, Hisham A, Alhadlaq MA. Synthesis, characterization, and antimicrobial activity of copper oxide nanoparticles. J Nanomate. 2014;2014:4.

Duran N, Durán M, de Jesus MB, Seabra AB, Fávaro WJ, G. Nakazato G. Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity. Nanomed. 12 (3), 2016;789–799.

Hessien M, Da’na E, Taha A. Phytoextract assisted hydrothermal synthesis of ZnO–NiO nanocomposites using neem leaves extract. Ceram. Int. 47 (1), 2021;811-816.

Ye Y, Cota-Ruiz K, Hernández-Viezcas JA, Valdés C, Medina-Velo IA, Turley RS, J.R. Peralta-Videa JR, Gardea-Torresdey JL. Manganese nanoparticles control salinity-modulated molecular responses in Capsicum annuum (L.) through Priming: A sustainable approach for agriculture. ACS Sustain. Chem. Eng. 8(3), 2020;1427–1436.

Prashanth GK, Prashanth PA, Nagabhushana BM. Comparison of anticancer activity of biocompatible ZnO nanoparticles prepared by solution combustion synthesis using aqueous leaf extracts of Abutilon indicum, Melia azedarach and Indigofera tinctoria as biofuels. Artificial Cells, Nanomed. Biotechnol. 46: 2018; 968–979.

Ghisalberti EL. Lantana camara L. (Verbenaceae). Fitoterapia. 71 (5), 2000; 467-486.

Gentle CB, Duggin JA, Allelopathy as a competitive strategy in persistent thickets of Lantana camara L. in three Australian forest communities. Plant Ecol. 132 (1), 1997;85-95.

Mittal AK, Chisti Y, UC. Banerjee UC. Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv. 2013;31:346–356.

Asemani M, Anarjan N. Green synthesis of copper oxide nanoparticles using Juglans regia leaf extract and assessment of their physico-chemical and biological properties. Green Process. Synth. 8, 2019; 557-567.

Prasad, K. S., Patra, A., Shruthi, G., & Chandan, S. (2017). Aqueous extract of Saraca indica leaves in the synthesis of copper oxide nanoparticles: finding a way towards going green. J Nanotech. 2017. Article ID 7502610, (6) doi.org/10.1155/2017/7502610

Islam MS, Lucky RA. A study on different plants of Apocynaceae family and their medicinal uses. J. Pharm. Res. 4(1), 2019; 40-44.

Inchulkar RS, Yuvraj KC, Nagendr S, Kamal S, Kewat ML, Scope of Agadtantra (Ayurvedic toxicology) in environmental pollution wsr to Janpadodhvansa & Dushivisha: A Review. Arch. Pharma Pract.10 (2), 2019; 81-88.

Kasana RC, Singh SK, Kumar P. Antifungal activity of copper oxide nanoparticles against the fungal pathogens isolated from arid environment, Jodhpur. Int. J. Curr. Microbiol. App. Sci. 9 (11), 2020; 901-909

Khan MR, Ahamad F, TF. Rizvi TF.Effect of nanoparticles on plant pathogens. in Advance in Phytonanotech. Ghorbanpour, M., Wani, S.H. (Eds.) Academic Press, Amsterdam, 2019; 215–240.

Sur DH, Mukhopadhyay M. Role of zinc oxide nanoparticles for effluent treatment using Pseudomonas putida and Pseudomonas aureofaciens. Bioproc. Biosyst. Eng. 42 (2), 2019;187–198.

Gopinath V, Priyadarshini S, Al-Maleki A, Alagiri M, Yahya R, Saravanan S. In vitro toxicity, apoptosis and antimicrobial effects of phyto-mediated copper oxide nanoparticles. RSC Adv. 6 (112), 2016;110986–110995.

Jayachandran, A., Aswathy, T. R., & Nair, A. S. (2021). Green synthesis and characterization of zinc oxide nanoparticles using Cayratia pedata leaf extract. Biochemistry and Biophysics Reports, 26, 100995.

Mohd. Yusof H, Rahman A, Mohamad R, UH Zaidan UH, Samsudin AA. Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Sci. Rep. 10 (1), 2020; 1-13.

Gaba S, Rai AK, Varma, A, Prasad A, Goel A. Biocontrol potential of mycogenic copper oxide nanoparticles against Alternaria brassicae. Front. Chem. 2022;10,966396.

N.A. Dhas, C.P. Raj, A. Gedanken, Synthesis, characterization, and properties of metallic copper nanoparticles. Chem. Mater. 10(5), 1998, 446–145.

Santhosh JK, Kumar SV, Rajesh KS. Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resource-Efficient Technol. 3(4), 2017; 459-465.

Singh S. Green synthesis and characterization of copper nanoparticles using Madhunashini leaf extract and evaluation of its antibacterial property. Int. J. Innov. Res. Sci. Eng. Technol. 2018;13 (24), 4364.

Sundaramurthy N, Parthiban C. Biosynthesis of copper oxide nanoparticles using Pyrus pyrifolia leaf extract and evolve the catalytic activity. Int. Res. J. Eng. Technol. 2 (6), 2015; 332-338.

Noor S, Shah ZZ, Javed A, Ali A, Hussain SB, Zafar SA. A fungal based synthesis method for copper nanoparticles with the determination of anticancer, antidiabetic and antibacterial activities. J. Microbiol. Methods. 174, 2020;105966.

Salari Z, Ameri A, Forootanfar H, Adeli-Sardou M, Jafari M, Mehrabani, Shakibaie, M. Microwave-assisted biosynthesis of zinc nanoparticles and their cytotoxic and antioxidant activity. J. Trace. Elem. Med. Biol. 39, 2017;116-123.

Jamdagni P, Khatri P, Rana JS. Green synthesis of zinc oxide nanoparticles using flower extract of Nyctanthes arbor-tristis and their antifungal activity. J. King Saud Univ. Sci. 30, 2018;168–175.

Ingle AP, Gupta, I. Role of Metal‐Based Nanoparticles in Plant Protection. Nanotechnology in Plant Growth Promotion and Protection: Recent Advances and Impacts, 2021; 220-238.

Abdelaziz AM, Salemm SS, Khalil A, El-Wakil DA, Fouda HM, Hashem AH, Potential of biosynthesized zinc oxide nanopar.ticles to control Fusarium wilt disease in eggplant (Solanum melongena) and promote plant growth. BioMetals. 20(101), 2022; 601–616.

Abdelghany T, Alharbi AA, Al-Rajhi A.M. Suppression application of copper oxide nanoparticles for wilt-inducing Fusarium equiseti in wheat 2021.

Pariona N, Mtz-Enriquez AI, Sánchez-Rangel D, Carrión G, Paraguay-Delgado F, Rosas-Saito G. Green-synthesized copper nanoparticles as a potential antifungal against plant pathogens. RSC Adv. 9 (33), 2019;18835–18843.

Kelly SA, Havrilla CM, Brady TC, Abramo KH, ED Levin ED. Oxidative stress in toxicology: established mammalian and emerging piscine model systems. Environ. Health Perspect. 1998;106:375–384.

Leroch M, Kretschmer M, M. Hahn M. Fungicide resistance phenotypes of Botrytis cinerea isolates from commercial vineyardsin south west Germany. J. Phytopathol. 159 (1)2011;63–65.




DOI: https://doi.org/10.37591/rrjols.v13i3.3777

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