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Preliminary Phytochemical Screening, Antioxidant and antibacterial activity of Helicteres isora leaf extracts.
Abstract
Objective: To evaluate phytochemical screening, Anti-oxidant and anti-bacterial activity of Helicteres isora leaf extracts. Methods: Petroleum ether, chloroform, acetone, ethanol and hydroalcoholic extracts of leaves were used for the phytochemical screening. Anti-oxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (No) radical scavenging tests; antibacterial activity was assessed using disc diffusion techniques. Results: The percentage yields for the petroleum ether, chloroform, acetone, ethanol and the hydroalcoholic extract were 1.29%, 1.76%, 1.41%, 2.18% and 3.23% w/w, respectively. H. isora leaf extract produced favourable results for sterols and flavonoids during the initial phytochemical screening. The DPPH radical-scavenging activity of the concentrations of ethanolic and hydroalcoholic extracts, and L ascorbic acid needed for 50% inhibition free radicals were found to be 84.02 µg/mL, 76.05 µg/mL and 54.96 µg/ml, respectively. The inhibition of No radicals generated from sodium nitroprusside at physiological pH by the ethanolic and hydroalcoholic extracts and L-ascorbic acid was observed at concentrations of 85.05 µg/mL, 74.03 µg/mL, and 51.69 µg/mL, respectively. Conclusions: The hydroalcoholic extract's observed antibacterial and antioxidant properties may be due to the phytochemical components of these substances
References
Sen, S. Chakraborty, R. The role of antioxidants in human health. In: Silvana A, Hepel M, editors. Oxidative Stress: Diagnostics, Prevention, and Therapy (ACS symposium series) Washington DC: American Chemical Society.2011; Pp. 1–37.
Li, H.Y. Hao Z.B. Wang X.L. Huang L. Li J.P. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresource Technology.2009; 100: 970–974.
Meghashri, S. Vijay Kumar H. Gopal S. Antioxidant properties of a novel flavonoid from leaves of Leucasaspera. Food Chemistry.2010;122: 105–110.
Roy, N. Laskar R.A. Sk I. Kumari D. Ghosh T. Begum N.A. A detailed study on the antioxidant activity of the stem bark of Dalbergia sissoo Roxb., an Indian medicinal plant. Food Chemistry 2011; 126: 1115–1121.
Srinivasan R.M. Chandraseka J.N. Nanjan M.S. Suresh B. Free radical Scavenging activity of Ipomoea obscura (L.) Ker-gawl. Journal of Natural Remedies2007;7(2): 184–88.
Kirtikar, K.R. Basu, B.D. An ICS, Indian medicinal plants. Vol. 1, Dehradun: International Book distributors.1995; Pp. 371–372.
Kirtikar KR basu BD. Indian Medicinal Plants. Dehradun, India: International Book Distributions, 1995; 1(2): 370–372.
Chopra RM. Rmnayar Chopra IC. Glossary of Indian Medicinal Plants. New Delhi: National Institute of Science Communication Council of Scientific and Industrial Research. 1956; 226–227.
Tibrewal R. Anti-diabetic & antioxidant studies of Helictere isora roots international. Journal of Pharmaceutical and Biological Science Archive, 2017; 5(1): 23–29.
Kumar Singh AK. Plant profile. phytochemistry and pharmacology of Avartani (Helicteres isora Linn.)
Gopalasathees kumar K. significant role of soxhlet extraction process in phytochemical research. Mintage Journal of Pharmaceutical & Medical Sciences.2018;7(Suppl1): 43–47.
Raja AXV Sama K. IJPRD. 2011; 3(12):136
Harborne JB. Phytochemical methods. London: Chapman and Hall, Ltd; 1973. p. 49–188. Ikan, R.,
(1981): Natural products: A laboratory guide, Academic Press, London.
Trease GE. Evans WC. Pharmacognsy. 11th ed: Brailliar Tiridel Can. Macmillan Publishers; 1989.
Ajaiyeobu EO. Afr J Biomed Res. 2002; 5:125.
Benmehdi H. et al. J Mater Environ Sci. 2012;3(2):320–237
Badami S. prakash O. Dongre S.et al. In vitro antioxidant properties of Solanum pseudocapsicum leaf extracts. Indian Journal of Pharmacology, 2005, 37(4): 251–252.
Rajeshwar Y. Kumar GPS. Gupta M. et al. Studies on in vitro antioxidant activities of methanol extract of Mucuna pruriens (Fabaceae) seeds. European Bulletin of Drug Research. 2005;13(1): 31–39.
Suthar M. Rathore GS. Pareek A. Antioxidant and antidiabetic activity of Helicteres isora (L) fruits. Indian Journal of Pharmaceutical Sciences, 2009;71(6): 695–699.
Venkatesh S, Sailaxmi K, Reddy BM, et al. antimicrobial activity of Helicteres isora root. Indian Journal of Pharmaceutical Sciences, 2007; 69(5): 687–689.
Bhalodia N. Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistula: an ethnomedicinal plant. Journal of Advanced Pharmaceutical Technology & Research, 2011;2(2):104–109.
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