Effect of IBA and BAP on in vitro Plant Regeneration of Mint

Abstract

Abstract

An experiment was conducted in the tissue culture laboratory of the department of horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur-1706, from March 2013 to February 2014 for in vitro regeneration of mint (Mentha sp) plantlets using different growth regulators in full strength MS media. Shoot tip was used as explant using three levels of BAP (1.0, 2.0 and 3.0 mg/l) and IBA (1.0, 2.0 and 3.0 mg/l) along with control for plantlet regeneration. Results revealed that shoot initiation (13.9 days) was earlier when 2.0 mg/l of IBA was used, which was statistically similar with other treatments of BAP except the control (34.8 days). In case of IBA, the lowest time required for root initiation when 1.0 mg/l was used (30.0 days). Regarding number of shoots and roots per explants, combination of 1.0 mg/l of both BAP and IBA showed the best performance compared to other treatments at different days after inoculation. Therefore, for in vitro plantlet regeneration of mint, combinations of 1.0 mg/l of both BAP and IBA may be used with the explant shoot tip.

Keywords: Regulators, in vitro, mint regeneration

Cite this Article

Kazi Tahmina Akter, Md. Azizul Hoque. Effect of IBA and BAP on in vitro Plant Regeneration of Mint. Research & Reviews: Journal of Crop Science and Technology. 2015; 4(3): 1–10p.