In vitro Antibacterial Activity of Dietary Spice Trigonella foenum-graecum and Syzygium aromaticum

Abstract

Multi-drug resistant strains of bacteria are broadly distributed in hospitals and are progressively being isolated from community. The aim of the contemporary study was to govern the potential of spices Trigonella foenum-graecum (Fenugreek) and Syzygium aromaticum (Clove) as antibacterial mediators against drug resistant and virulent strains of gram negative pathogens quarantined from urinary pathway infections. Twelve bacterial isolates from urinary tract infections were studied for their ability to produce virulence phenotypes like hemolysin, biofilms, gelatinases, hydrophobic nature and penicillinases. Antibacterial susceptibility testing was done. Out of the total isolates tested 67% of E. coli and 60% of Klebsiella Pneumoniae sp were multi-drug resistant. Penicillinase production as tested by filter paper iodometric method and biofilm production on Congo red plates were the virulence features exhibited by maximum number of E. coli and Klebsiella Sp. Among the ethanol methanol, acetone and chloroform removes of fenugreek and clove, chloroform extracts bequeathed an extreme diameter of zone of inhibition with E. coli, Klebsiella Sp and Pseudomonas sp verified. Minimum inhibitory concentration (MIC) for clove and fenugreek extracts against the clinical isolates was obtained in the range of 1.25–2.5 mg/ml. Synergistic activity of clove and fenugreek extracts against the isolates were tested. The inhibitory activity of fenugreek either increased or remained the same in presence of clove where as there was no such enhancement in clove in presence of fenugreek extracts i.e., inhibitory activity remained the same. 

Keywords: Trigonella foenum-graecum, Syzygium aromaticum, E.coli, Klebsiella Sp

Cite this Article

Rinzi Bhutia, Vishaka Labar, Annapurna YVS. In vitro Antibacterial Activity of Dietary Spice Trigonella foenum-graecum and Syzygium aromaticum. Research and Reviews: Journal of Herbal Sciences. 2016; 5(1): 6–11p.