Evaluation of Different Essential Oils for Antifungal Activity on Postharvest Decay of Avocado (Persea Americana Mill.) of Sikkim, India

Authors

  • Barsha Sunar
  • Saurav Das
  • Pinkey Lhamu Lepcha
  • Mingma Thundu Sherpa
  • Laxuman Sharma

Abstract

Commercially available essential oils of Basil oil, Clove oil, Citronella oil, Ginger oil and methanol extracted fern oil, Artemisia oil, and Stinging nettle oil were tested for growth inhibitions of two fungi isolated from infected avocado fruit from Sikkim, India which were identified as Fusarium sp. and  Penicillium digitatum on the basis of colony morphology and microscopic examination. Inhibitory effect of essential oils was investigated by well diffusion method by applying different concentration of essential oils along with positive control taken as Bavistin and negative control deionized water. The results demonstrated that Fern and Clove oil showed the considerable effects against both the fungus. The minimum inhibitory concentration (MIC) of essential oils was also evaluated by spore germination assay and the result demonstrated that the MIC value of Fern oil against Penicillium digitatum and Fusarium sp. were 400 ppm. Similarly, Clove oil showed MIC value of 600 ppm against both the isolated fungus. Furthermore, in vivo analysis of fern and clove oil showed that the reductions of disease incidence in avocado fruit were 40% and 45% at a concentration of 400 ppm respectively. Our results suggested that the Fern oil (Gymnocarpium dryopteris) and Clover oil (Syzygium aromaticum) were a better option against chemical fungicides to prevent postharvest loss of Avocado fruit.

 

Keywords: Postharvest decay, avocado fruit, essential oil, antifungal activity

Cite this Article

Barsha Sunar, Saurav Das, Pinkey Lhamu Lepcha, Mingma Thundu Sherpa, Laxuman Sharma. Evaluation of Different Essential Oils for Antifungal Activity on Postharvest Decay of Avocado (Persea Americana Mill.) of Sikkim, India. Research & Reviews: A Journal of Life Sciences. 2019; 9(2): 44–51p.

Downloads

Published

2019-06-03

Issue

Vol. 9 No. 2 (2019)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.