In Vitro Propagation of Cassia alata L and Its Secondary Metabolites

Abstract

Cassia alata (L.) is traditionally used to treat various fungal infections, traditionally used as a remedy for poisonous bites. The objectives of the present study were to conservation of Cassia alata through the plant tissue culture and comparative of its secondary metabolite with field cultivated plant as well as in vitro. In micropropagation, multiple shoots were induced by the nodal region of the plant on Murashige and Skoog medium containing plant growth regulators such as 6-benzylaminopurine, Kinetin, and indole-3-acetic acid. The multiplication were achieved in the combination of 6-benzylaminopurine with naphthalene acetic acid or indole-3-acetic acid. The present investigation has observed that maximum number and shoots per explants was obtained on MS medium with 6-benzylaminopurine at 1.0 mg/l concentration and the roots were proliferated in the combination of 6-benzylaminopurine (1.0 mg/l) and indole-3-acetic acid (1.0 mg/L) concentration. Root induction was achieved on MS medium supplemented with IAA. The well-rooted in vitro propagated Cassia alata was successfully transferred to the greenhouse for acclimatization. Field cultivated plant was propagated through seedlings in sand. A 5 month propagated plant leafs were successfully collected for comparative study of total phenol and total flavonoids content. In the present secondary metabolite estimation has confirmed that total flavonoids (48.52  ± 0.79) was found to be maximum in micropropagated plant and total phenol content (50.0 ± 1.7) was found to be maximum in the field cultivated plant.

Keywords: 6-benzylaminopurine, comparative, Murashige and Skoog medium, phenol, flavonoids, propagation