Cost Effective Method of Protease Production in Solid State Fermentation Using Combined Substrate Corn Cob and Lentil Husk

Authors

  • Monika Prakash Rai
  • Subhasha Nigam

Abstract

An alkalophilic Bacillus cereus strain was found to produce a thermal and pH tolerant extracellular alkaline protease which showed very good compatibility and stability with commercial laundry detergents. Considering important industrial application of B. cereus protease, its production needs to be enhanced. Different agro-industrial waste products, viz., wheat bran, lentil husk, green gram husk and corn cob were used as substrates for the production of B. cereus protease by solid state fermentation (SSF). The production of alkaline protease in SSF was compared with that in submerged fermentation (SmF) by using the same substrates. This comparative study revealed that production of B. cereus protease is significantly higher in SSF than in SmF. Among all the substrates used, corn cob followed by lentil husk showed best production in SSF. Combined effect of corn cob and lentil husk was examined when mixed in 1:1 ratio. The production of protease was enhanced almost double in comparison to individual substrate. Other important parameters like inoculum size, substrate concentration, fermentation time, incubation temperature and moisture content were optimized to get maximum protease production in SSF. Maximum protease activity of 580.80 u/mL/min (4640 U/g solid substrate) was obtained in SSF when initial moisture content was maintained at 60%, 3 g of corn cob and lentil husk were used in 1:1 ratio, 2.0–2.5 mL (2 mg/mL) of inoculum was added and culture was incubated at 30 °C for 7 days.
Keywords: Bacillus cereus, alkaline protease, solid state fermentation, corn cob, lentil husk

Downloads

Published

2018-05-22

Issue

Vol. 3 No. 1 (2013)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.