Comparative Study of Extraction and Characterization of Chitosan by Chemical and Biological Method from the Crab Shell Waste and Analyzing its Anticancer Activity

Abstract

Crab shell waste causes environmental pollution, So it is in need to process the waste into useful chitosan The crab shell waste act as an alternative for chitosan production The chitosan molecule appears to be a suitable polymeric complex for many biomedical applications. Chitosan is an amino polysaccharide and a versatile, natural polysaccharide. The specific objective of this study is to compare chitosan from the exoskeleton of freshwater crab obtained by two methods such as chemical and biological method. The extracted chitosan from both the methods are purified and characterized the quality of chitosan which includes parameters such as pH, moisture content, solubility, loss of drying and FT-IR (Fourier Transform Infrared Spectroscopy) Further the presence of components in the chitosan is identified by GC-MS (Gas Chromatography–Mass spectrometry). Finally, the bioactivity of chitosan is determined by anticancer activity. The anticancer potential of the chitosan on HT29 and Hep2 cell lines was determined by MTT assay. The results on MTT assay readings assert the significant inhibitory action of chitosan on HT29 and Hep2 cell lines.

Keywords: Crab shell, chitosan, extraction, chemical method, biological method, purification, characterization, FT-IR, GC-MS, anticancer activity

Cite this Article

D. Sumalatha S. Albina Jannet. Comparative Study of Extraction and Characterization of Chitosan by Chemical and Biological Method from the Crab Shell Waste and Analyzing its Anticancer Activity. Research & Reviews: Journal of Veterinary Science and Technology. 2019; 8(1): 22–32p.