Tissue Culture Studies on Bacopa monnieri: A Review

Abstract

Abstract
As the plants goes endangered due to overexploitation and anthropogenic activities, there is necessity to develop a technique to conserve them—micropropagation is one of them. To conserve Bacopa—a high economic value contained medicinal plant—different tissue culture modes are used. The growth agents were used at different concentration and high growth of regeneration was found. This review highlights the recent development and achievements made for the multiple shoots regenerated from different explants. As a result, the induction of multiple shoots from nodal segments were found highest in MS medium with 0.5 mg/L BAP + 2.0 mg/L Kinetin (Kn) and 0.5 mg/L Kn+1.0 mg/L BAP. Highest rooting was recorded on MS medium supplemented with 2.0 mg/L IBA. In leaf petiole, best growth was observed in MS medium supplemented with 0.25 mg/L 2,4-D+ 0.5 mg/L Kn and 0.25 mg/L 2,4-D+ 0.1 mg/L BAP.
Keywords: Micropropagation, Explants, Propagule, MS Medium