Chitosan-conjugated GnRH Nanoparticles with AFM Appearance

Abstract

Nanotechnology can provide new tools for molecular and cellular biology, biotechnology, veterinary physiology, reproduction, detection of pathogens and many more. There are several areas such as science, engineering of agriculture, animal and food systems where nanotechnology could be applied. Application of nanotechnology can be employed in the development of sustained release systems of molecules such as hormones, vitamins, antibiotics, antioxidants and nucleic acids. Longer lasting surges in Gonadotropin hormone (GtH) blood levels can be attained by using multiple injections of Gonadotropin-releasing hormone (GnRH) or analogues, which unavoidably involves repeated handling of animals resulting in increased cost, time, labor and avoidable stress to both the animals and handler. However, since GnRH having very short half-life undergoes proteolysis within a few minutes the newer nanotechnology has come into place for effective delivery of hormones. The current research work was undertaken to induce estrus synchronization (ES) in Kilakarsal ewes, using chitosan-nanoconjugated GnRH and to assess its efficacy and economical feasibility. Chitosan nanoparticles were prepared by ionic crosslinking of chitosan solution with tripolyphosphate (TPP) anions. Nanoparticles were characterized by adopting advanced microscopic technique using Atomic Force Microscope (AFM).

Keywords: Estrus synchronization (ES), nanoconjugated Gonadotropin-releasing hormone (GnRH), ewes, atomic force microscope (AFM), ionic gelation

Cite this
Article
M. Sundara Vinayaki . Chitosan c onjugated
GnRH Nanoparticles with AFM
Appearance Research & Reviews: Journal
of Veterinary Science and Technology. 2019;
8(3): 3 5 p.