Influence of Geography and Gestation on Breast Milk Microbiota

Jasmina Javaid Shah, Meenakshi Anad

Abstract


Breast milk serves as a critical source of nutrition and immune factors crucial for infant health and development. This study sought to delve into the intricate relationship between geographical location, gestational age, and the microbiota present in breast milk, aiming to shed light on their collective impact on infant well-being. Twenty breast milk samples were meticulously gathered from lactating mothers at Civil Hospital Mohali, representing a diverse cross-section of individuals. Through rigorous laboratory analyses, the microbial composition of these samples was thoroughly scrutinized, allowing for a comprehensive assessment of the interplay between geographical factors and gestational age on breast milk microbiota. Remarkably, lactobacillus spp. emerged as the dominant bacterial species across the samples, echoing previous research showcasing the probiotic potential of breast milk. However, the presence of other bacterial strains, including streptococcus spp., Enterococcus spp., and Bifidobacterium spp., underscored the rich diversity inherent in breast milk microbiota. Furthermore, the findings highlighted the nuanced influence of geographical location and gestational age on the microbial landscape of breast milk. Factors such as regional variations and the duration of gestation appeared to exert discernible effects on the composition and abundance of specific microbial species, thereby shaping the overall microbial profile of breast milk. In conclusion, this study underscores the multifaceted role of breast milk as a dynamic reservoir of beneficial microbes, with geographical location and gestational age acting as influential determinants of its microbial composition. The dominance of lactobacillus spp. underscores the potential immunological benefits conferred by breast milk, underscoring its pivotal role in promoting infant health and development.


Keywords


Breast Milk, Microbiota, Gram Staining, Lactobacillus, Staphylococcus, Streptococcus

References


Meng X, Dunsmore G, Koleva P, Elloumi Y, Wu RY, Sutton RT, Ambrosio L, Hotte N, Nguyen V, Madsen KL, Dieleman LA. The profile of human milk metabolome, cytokines, and antibodies in inflammatory bowel diseases versus healthy mothers, and potential impact on the newborn. Journal of Crohn's and Colitis. 2019 Mar 30;13(4):431-41.

Stuebe, Alison. "The risks of not breastfeeding for mothers and infants." Reviews in obstetrics and gynecology 2.4 (2009): 222.

Lodge CJ, Tan DJ, Lau MX, Dai X, Tham R, Lowe AJ, Bowatte G, Allen KJ, Dharmage SC. Breastfeeding and asthma and allergies: a systematic review and meta‐analysis. Acta paediatrica. 2015 Dec; 104:38-53.

Luccioli S, Zhang Y, Verrill L, Ramos-Valle M, Kwegyir-Afful E. Infant feeding practices and reported food allergies at 6 years of age. Pediatrics. 2014 Sep 1;134(Supplement_1):S21-8.

Jwa SC, Fujiwara T, Kondo N. Latent protective effects of breastfeeding on late childhood overweight and obesity: a nationwide prospective study. Obesity. 2014 Jun;22(6):1527-37.

Wejryd E, Martí M, Marchini G, Werme A, Jonsson B, Landberg E, Abrahamsson TR. Low diversity of human milk oligosaccharides is associated with necrotising enterocolitis in extremely low birth weight infants. Nutrients. 2018 Oct 20;10(10):1556.

Longini M, Tataranno ML, Proietti F, Tortoriello M, Belvisi E, Vivi A, Tassini M, Perrone S, Buonocore G. A metabolomic study of preterm and term human and formula milk by proton MRS analysis: Preliminary results. The Journal of Maternal-Fetal & Neonatal Medicine. 2014 Oct 1;27(sup2):27-33.

Kumar H, Du Toit E, Kulkarni A, Aakko J, Linderborg KM, Zhang Y, Nicol MP, Isolauri E, Yang B, Collado MC, Salminen S. Distinct patterns in human milk microbiota and fatty acid profiles across specific geographic locations. Frontiers in microbiology. 2016 Oct 13;7:1619.

Kumar H, Du Toit E, Kulkarni A, Aakko J, Linderborg KM, Zhang Y, Nicol MP, Isolauri E, Yang B, Collado MC, Salminen S. Distinct patterns in human milk microbiota and fatty acid profiles across specific geographic locations. Frontiers in microbiology. 2016 Oct 13;7:1619.

Boix-Amorós A, Collado MC, Mira A. Relationship between milk microbiota, bacterial load, macronutrients, and human cells during lactation. Frontiers in microbiology. 2016 Apr 20;7:492.

Munblit D, Boyle RJ, Warner JO. Factors affecting breast milk composition and potential consequences for development of the allergic phenotype. Clinical & Experimental Allergy. 2015 Mar;45(3):583-601.

Chung MY. Factors affecting human milk composition. Pediatrics & Neonatology. 2014 Dec 1;55(6):421-2.

Ballard O, Morrow AL. Human milk composition: nutrients and bioactive factors. Pediatric Clinics. 2013 Feb 1;60(1):49-74.

Yuhas R, Pramuk K, Lien EL. Human milk fatty acid composition from nine countries varies most in DHA. Lipids. 2006 Sep;41(9):851-8.

Gómez-Gallego C, Morales JM, Monleón D, Du Toit E, Kumar H, Linderborg KM, Zhang Y, Yang B, Isolauri E, Salminen S, Collado MC. Human breast milk NMR metabolomic profile across specific geographical locations and its association with the milk microbiota. Nutrients. 2018 Sep 21;10(10):1355.

Lordan C, Roche AK, Delsing D, Nauta A, Groeneveld A, MacSharry J, Cotter PD, van Sinderen D. Linking human milk oligosaccharide metabolism and early life gut microbiota: bifidobacteria and beyond. Microbiology and Molecular Biology Reviews. 2024 Jan 11:e00094-23.

Li SW, Watanabe K, Hsu CC, Chao SH, Yang ZH, Lin YJ, Chen CC, Cao YM, Huang HC, Chang CH, Tsai YC. Bacterial composition and diversity in breast milk samples from mothers living in Taiwan and Mainland China. Frontiers in microbiology. 2017 May 30;8:965.

Pannaraj PS, Li F, Cerini C, Bender JM, Yang S, Rollie A, Adisetiyo H, Zabih S, Lincez PJ, Bittinger K, Bailey A. Association between breast milk bacterial communities and establishment and development of the infant gut microbiome. JAMA pediatrics. 2017 Jul 1;171(7):647-54.

Maynard CL, Elson CO, Hatton RD, Weaver CT. Reciprocal interactions of the intestinal microbiota and immune system. Nature. 2012 Sep 13;489(7415):231-41.

Jost T, Lacroix C, Braegger C, Chassard C. Impact of human milk bacteria and oligosaccharides on neonatal gut microbiota establishment and gut health. Nutrition reviews. 2015 Jul 1;73(7):426-37.

Blewett HJ, Cicalo MC, Holland CD, Field CJ. The immunological components of human milk. Advances in food and nutrition research. 2008 Jan 1;54:45-80.

Khodayar-Pardo P, Mira-Pascual L, Collado MC, Martínez-Costa C. Impact of lactation stage, gestational age and mode of delivery on breast milk microbiota. Journal of Perinatology. 2014 Aug;34(8):599-605.

Moltu SJ, Blakstad EW, Strømmen K, Almaas AN, Nakstad B, Rønnestad A, Brække K, Veierød MB, Drevon CA, Iversen PO, Westerberg AC. Enhanced feeding and diminished postnatal growth failure in very-low-birth-weight infants. Journal of pediatric gastroenterology and nutrition. 2014 Mar;58(3):344.

Korpela, Katri. "mare: microbiota analysis in R easily." R package version 1.10.5281 (2016).

Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010 Oct 1;26(19):2460-1.




DOI: https://doi.org/10.37591/rrjodst.v12i3.3828

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