Isolation and Characterization of Lactobacillus spp. of Human Origin for Studying their Prevalence

Authors

  • Monica Puniya
  • KPS Sangu
  • Aarti Bhardwaj
  • Sanjay Kumar
  • Tejpal Dhewa

DOI:

https://doi.org/10.37591/rrjodst.v2i3.857

Abstract

A total of 15 samples of healthy human feces were collected for the isolation of lactobacilli using lactobacillus selection MRS agar. Thirty-eight colonies were randomly picked based on colonial morphology. All the isolates were subjected to cell morphology, physiology and an array of biochemical characterization. The isolates showed different growth patterns at different temperatures (15 and 45 °C), oxygen and at different concentrations of NaCl (2.0, 4.0 and 6.5%). On the basis of physiological tests and sugar utilization pattern, all the thirty eight isolates were confirmed to the different species of Lactobacillus (i.e., Lactobacillus plantarum (4), Lactobacillus casei (15), Lactobacillus rhamnosus (2), Lactobacillus fermentum (4), and Lactobacillus acidophilus (10), Lactobacillus helveticus (3). Among isolates Lactobacillus casei was found to be prevalent in the human gut.
Keywords: Lactobacilli, biochemical characterization, human gut

Downloads

Published

2018-05-21

Issue

Vol. 2 No. 3 (2013)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.