In vitro Responses of Indigofera tinctoria Linn. (Neela Amari)

Abstract

Indigofera tinctoria is an important medicinal plant of South and South-East Asia. These plants showed a low germination rate and scanty rooting of seedlings in conventional propagation. The tender leaves showed callus formation in MS medium with single auxin [Naphthalene Acetic Acid (NAA); 2, 4 Dichloro phenoxy Acetic acid (2, 4-D)]. Lower concentration of auxin showed better result. The explants showed callusing frequency of 95%. Even though both the auxins induced callus, they showed a difference in the days for response. Rhizogenesis along with callus was noticed only in NAA supplemented medium. It was noticed that NAA showed better result in case of initial callusing and also rhizogenesis.

Keywords: In vitro, rhizogenesis, callus, explants, NAA

Cite this Article

Swetha Thilak T, Indulekha P. In vitro Responses of Indigofera tinctoria Linn. (Neela Amari). Research & Reviews: Journal of Herbal Science. 2017; 6(1): 5–9p.