Variation in Activity of Lipase Immobilized on Chitosan and Alginate Nanoparticles by Changing Concentration of the Preparatory Reagents

Abstract

Chitosan and alginate nanoparticles were prepared by ionic gelation method. The concentrations of each of the preparatory reagents (for chitosan: acetic acid, chitosan, sodium sulphate, glutaraldehyde and for alginate: calcium chloride, alginate, glutaraldehyde) were altered to check their consequences. Lipase was chemically crosslinked to these nanoparticles. Enzymatic assay was carried out for every alteration separately. To visualize the changes in each reagent at every step a precise table is incorporated in the text. It can be clearly understood from the outcomes that optimum concentration of each preparatory reagent is mandatory for maximum activity as can be seen from the graphical representations. Minute permutations in certain reagents can cause major difference in the results. During the study, it was also observed that certain changes in concentration affect the structure and rigidity of the nanoparticles.

Keywords: Immobilization, glutaraldehyde, microspheres, nanospheres, ionic gelation

Cite this Article Devnani H, Bahadur A. Variation in Activity of Lipase Immobilized on Chitosan and Alginate Nanoparticles by Changing Concentration of the Preparatory Reagents. Research & Reviews: A Journal of Life Sciences. 2017; 7(3): 14–22p.