Isolation and Identification of Lactobacillus from Raw Buffalo Milk from the Local Region of Agra
Keywords:
Probiotic microbes, buffalo milk, Lactobacilli, IMViC, biochemical testsAbstract
The term ‘Probiotic’, literally means, ‘for life’ which describes “substances secreted by one microorganism stimulating the growth of another”. These are viable microorganisms which contribute to intestinal microbial balance and help to improve the gut health in humans and other animals. Probiotics have been used in animal feed industry as a food supplements. They are also used to manufacture the fermented dairy products widely. The most common among them are Bifidobacteria and Lactic acid bacteria (LAB) such as Lactobacilli, Lactococci and Streptococci. Probiotic microbes are normally present in milk and milk products such a yogurt, cheese butter milk, butter, etc. So, a study was carried out to isolate the Lactobacilli from raw buffalo milk samples. The isolated samples were identified by using biochemical tests such as IMViC and other sugar fermentation tests. The results of the study indicated the predominance of Lactobacilli in almost all milk samples collected.
References
Hoque MZ, Akter F, Hossain KM, Rahman MSM, Billah MM, Islam KMD. Isolation, Identification and Analysis of Probiotic Properties of Lactobacillus Spp. From Selective Regional Yoghurts. World Journal of Dairy & Food Sciences (WJDFS). 2010; 5(1): 39–46p.
Lilly DM, Stillwell RH. Probiotics: Growth-Promoting Factors Produced by Microorganisms. Sci. 1965; 147:
–748p.
Ogier JC, Son O, Gruss A, Tailliez P, Delacroix-Buchet A. Identification of Bacterial Microflora in Dairy Products by Temporal Temperature Gradient Gel Electrophoresis. J Appl Environ Microbiol. 2002; 68(8): 3691–701p.
Otles S, Cagindi O. Kefir: A Probiotic Dairy: Composition, Nutritional and Therapeutic Aspects. Pakistan J Nutr. 2003; 2(2): 54–59p.
Van-Niel CW, Feudtner C, Garrison MM, Christakis DA. Lactobacillus is Safe and Effective for Treating Children with Acute Infectious Diarrhoea. Pediatrics. 2002; 109(4): 678–684p.
Desmasures N, Bauin F, Guequen M. Microbiological Composition of Raw Milk from Selected Farms in the Cambert Region of Normandy. J Appl Microbiol. 1997; 83(1): 53–58p.
Mithun S, Dipak V, Sheela S. Isolation and Identification of Lactobacilli from Raw Milk Sample Obtained from Aarey Milk Colony. International Journal of Scientific and Research Publications (IJSRP). 2015; 5(4): 2250–3153p.
Shembil SS. Isolation of Lactic Acid Bacteria with Antimicrobial Activity. A Dissertation Submitted to Brac University in Partial Fulfillment of the Requirements for the Degree of Bachelor of Science in Biotechnology. 2016.
Tambekar DH, Bhutada SA. An Evaluation of Probiotic Potential of Lactobacillus Sp. from Milk of Domestic Animals and Commercial Available Probiotic Preparations in Prevention of Enteric Bacterial Infections. Recent Res Sci Technol. 2010; 2(10): 82–88p.
Narwade RB, Kasare JD, Choudhary SR. Isolation, Screening and Characterization of Lactobacilli from Cow Milk. Int J Agric Innov Res. 2015; 3(6): 2319–1473p.
Rizqiati H, Sumantri C, Noor RR, Damayanthi E, Rianti EI. Isolation and Identification of Indigenous Lactic Acid Bacteria from North Sumatra River Buffalo Milk. JITV. 2015; 20(2): 87–94p.
Shafakatullah N, Chandra M. Screening of Raw Buffalo’s Milk from Karnataka for Potential Probiotic Strains. Res J Recent Sci. 2014; 3(9): 73–78p.
Abbas MM, Mahasneh AM. Isolation of Lactobacillus Strains with Probiotic Potential from Camel’s Milk. Afr J Microbiol Res. 2014; 8(15): 1645–1655p.
Downloads
Published
Issue
Section
License
Declaration and Copyright Transfer Form
(to be completed by authors)
I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.
- I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
- I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
- I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
- I/We also agree to the authorship of the article in the following order:
Author’s name
1. ________________
2. ________________
3. ________________
4. ________________
| We Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal. |