Improvement of Yield of Bioactive Compounds and Antioxidant Activity of Moringa stenopetala Leaves by Optimizing Ultrasonic-assisted Extraction

Abstract

Ultrasonic-assisted extraction (UAE) parameters were optimized in this study using response surface methodology (RSM) to improve the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays of Moringa stenopetala leaves. According to the results, the optimal UAE parameters were extraction time (26 min), ethanol concentration (68%), and solvent-to-sample ratio (42 mL/g). Based on these optimal parameters, the predicted TPC and TFC values were 45.7 mg GAE/g-dw and 41.36 mg CE/g-dw, respectively. The antioxidant activities of the extracts were assessed using DPPH and ABTS assays and the IC₅₀ values were 29.23 µg/mL and 13.37 µg/mL, respectively, whereas the experimental values of the UAE extracts using optimal parameters were 45.75 mg GAE/g-dw (TPC), 39.2 mg CE/g-dw (TFC), 30.62 µg/mL (DPPH), and 12.87 µg/mL (ABTS). These show that the experimental values were very close to the predicted values. Therefore, the optimized UAE is important for the extraction of M. stenopetala leaves with advantages of shorter time and lower cost.

Key words: Antioxidant activity, bioactive compounds, Moringa stenopetala, optimization, UAE

Cite this Article

Debebe Worku Dadi, Shimelis Admassu Emire, Asfaw Debella Hagos, et al. Improvement of Yield of Bioactive Compounds and Antioxidant Activity of Moringa stenopetala Leaves by Optimizing Ultrasonic-assisted Extraction. Research & Reviews: Journal of Food Science and Technology. 2018; 7(3):
1–11p.