Optimization of Bio-surfactant Production by Solid State Fermentation using Response Surface Methodology

Abstract

Optimization of bio—surfactant production was carried out. Response surface methodology (RSM) was used as a statistical optimization tool for the same. Potato peel, an agricultural residue was utilized as the solid state fermentation (SSF) media, which served as both carbon and nitrogen sources. A 25 full factorial central composite experimental design was employed followed by the analysis of variance. The variable factors were Time (days), Temperature (°C), pH, Substrate amount (g), Solution volume (mL) which accounted for five levels of variability (-α, -1, 0, +1, + α). The maximum drop in surface tension was recorded to be 36.428 mn /m, for the fermentation time being 4 days, temperature being 35°C, pH 8.99, with 4 g substrate and 3.99 ml solution.

Keywords: Biosurfactant, RSM, Surface Tension, SSF, Agricultural residue

Cite this Article
Soumik Banerjee, Sarmistha Basak, Uma Ghosh. Optimization of Bio-surfactant Production by Solid State Fermentation using Response Surface Methodology. Research & Reviews: Journal of Food Science and Technology. 2019; 8(2): 21–32p.