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Separation methods and application possibilities of pyrolysis bio-oil: a short review

Valeska Morgana Côrrea Batistella, Karolynne Sousa Gomes, Aline Dettmer, Jeferson Stefanello Piccin


Bio-oil is a renewable energy source and has many compounds of industrial interest, but it also has undesirable characteristics such as high viscosity, corrosivity and high oxygen content, so separation means are necessary according to its application. There are many methods for the separation of bio-oil, so this review addresses the most used extraction techniques, among them: liquid-liquid, distillation, supercritical fluid extraction, catalytic cracking and adsorption. In addition, pyrolysis oils have promising characteristics for several applications, such as biodiesel production and also, due to their fungicidal effect, have been studied as pesticides applied in agriculture, which can positively impact food production, due to the reduction of water losses. food crops. The promising conditions and results reported in recent studies were presented, as well as possibilities for the application of bio-oil, with emphasis on the production of biodiesel and the possibility of using bio-oil as a fungicide agent. further studies on this topic, in order to contribute to scientific and technological development.


Bio-oil, Separation techniques, Applications.

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Hu X, Gholizadeh M. Progress of the applications of bio-oil. Renewable and Sustainable. Energy Review, 2020; 134, 110124p. doi: 10.1016/j.rser.2020.110124

Tagade A, Kirti N, Sawarkar AN. Bioresource Technology Reports Pyrolysis of agricultural crop residues: An overview of researches by Indian scientific community Pyrolysis Biochar Bio-oil Gas Soil additive Activated Organic acids Bio based chemicals. Bioresource Technology Reports., 2021; 15, 100761p. doi: 10.1016/j.biteb.2021.100761

Zhang L, Hu X, Li C, et al. Fates of heavy organics of bio-oil in hydrotreatment: The key challenge in the way from biomass to biofuel. Science of The Total Environment, 2021; 778, 146321p. doi: 10.1016/j.scitotenv.2021.146321

Gholizadeh M, Hu X, Liu QA mini review of the specialties of the bio-oils produced from pyrolysis of 20 different biomasses. Renewable and Sustainable Energy Reviews, 2019; 114, 109313 p. doi: 10.1016/j.rser.2019.109313

Bedmutha R, Booker CJ, Ferrante L, et al. Insecticidal and bactericidal characteristics of the bio-oil from the fast pyrolysis of coffee grounds. Journal of Analytical and Applied Pyrolysis, 2011; 90(2), 224–231p. doi: 10.1016/j.jaap.2010.12.011

Prabowo H, Edi M, Witjaksono W, et al. Activity of liquid smoke of tobacco stem waste as an insecticide on Spodoptera litura Fabricius Larvae. Jurnal Perlindungan Tanaman Indonesia, 2016; 20 (1), 22-27p. doi: 10.22146/jpti.16620

Naik SN, Goud VV, Rout P, et al. Production of first and second generation biofuels: A comprehensive review. Renewable and Sustainable Energy Reviews, 2010; 14(2), 578–597p. doi: 10.1016/j.rser.2009.10.003

Kumar R. A review on the modelling of hydrothermal liquefaction of biomass and waste feedstocks. Energy Nexus., 2022; 5, 100042 p. doi: 10.1016/

Hoang AT, Ong HC, Fattah IM, et al. Progress on the lignocellulosic biomass pyrolysis for biofuel production toward environmental sustainability. Fuel Processing Technology., 2021; 223, 106997 p. doi: 10.1016/j.fuproc.2021.106997

Ahmad M, Rajapaksha AU, Lim JE, et al. Biochar as a sorbent for contaminant management in soil and water: A review. Chemosphere., 2014; 99, 19–33p. doi: 10.1016/j.chemosphere.2013.10.071

Safdari MS, Amini E, Elham W, et al. Heating rate and temperature effects on pyrolysis products from live wildland fuels. Fuel., 2019; 242, 295–304p. doi: 10.1016/j.fuel.2019.01.040

Bridgwater AV. Review of fast pyrolysis of biomass and product upgrading. Biomass and Bioenergy., 2012; 38, 68–94p. doi: 10.1016/j.biombioe.2011.01.048

Drugkar K, Rathod W, Sharma T, et al. Advanced separation strategies for up-gradation of bio-oil into value-added chemicals: A comprehensive review. Separation and Purification Technology, 2022; 283, 120149 p. doi: 10.1016/j.seppur.2021.120149

Rocha KC, Alonso CG, Leal WGO, et al. Slow pyrolysis of Spirulina platensis for the production of nitrogenous compounds and potential routes for their separation. Bioresource Technology, 2020; 313, 123709 p. doi: 10.1016/j.biortech.2020.123709

Wei Y, Lei H, Wang L, et al. Liquid–liquid extraction of biomass pyrolysis bio-oil. Energy & Fuels, 2014; 28(2), 1207-1212 p. doi: 10.1021/ef402490s

Hu HS, Wu YL, Yang MD. Fractionation of bio-oil produced from hydrothermal liquefaction of microalgae by liquid-liquid extraction. Biomass and Bioenergy, 2018; 108, 487–500 p. doi: 10.1016/j.biombioe.2017.10.033

Wang Y, Wang S, Leng F, et al. Separation and characterization of pyrolytic lignins from the heavy fraction of bio-oil by molecular distillation. Separation and Purification Technology, 2015; 152, 123–132 p. doi: 10.1016/j.seppur.2015.08.011

Wang S, Wang Y, Leng F, et al. Stepwise enrichment of sugars from the heavy fraction of bio-oil. Energy & Fuels, 2016; 30(3), 2233-2239p. doi: 10.1021/acs.energyfuels.6b00039

Capunitan JA, Capareda SC. Characterization and separation of corn stover bio-oil by fractional distillation. Fuel, 2013; 112, 60–73 p. doi: 10.1016/j.fuel.2013.04.079

Feng Y, Meier D. Extraction of value-added chemicals from pyrolysis liquids with supercritical carbon dioxide. Journal of Analytical and Applied Pyrolysis, 2015; 113, 174–185 p. doi: 10.1016/j.jaap.2014.12.009

Cheng T, Han Y, Zhang Y, et al. Molecular composition of oxygenated compounds in fast pyrolysis bio-oil and its supercritical fluid extracts. Fuel, 2016; 172, 49–57 p. doi: 10.1016/j.fuel.2015.12.075

Chan YH, Yusup S, Quitain AT, et al. Extraction of palm kernel shell derived pyrolysis oil by supercritical carbon dioxide: Evaluation and modeling of phenol solubility. Biomass and Bioenergy., 2018; 116, 106–112 p. doi: 10.1016/j.biombioe.2018.06.009

Sedai B, Jin LZ, Nansi F, et al. Solid Phase Extraction of Bio-Oil Model Compounds and Lignin-Derived Bio-Oil Using Amine-Functionalized Mesoporous Silicas. ACS Sustainable Chemistry and Engineering, 2018; 6 (8), 9716–9724 p. doi: 10.1021/acssuschemeng.8b00747

Nam H, Choi J, Capareda SC. Comparative study of vacuum and fractional distillation using pyrolytic microalgae (Nannochloropsis oculata) bio-oil. Algal Research., 2016; 17, 87–96 p. doi: 10.1016/j.algal.2016.04.020

Valle B, Palos R, Bilbao J, et al. Role of zeolite properties in bio-oil deoxygenation and hydrocarbons production by catalytic cracking. Fuel Processing Technology, 2022; 227, 107130 p. doi: 10.1016/j.fuproc.2021.107130

Ibarra Á, Ita I, Azkoiti MJ, et al. Catalytic cracking of raw bio-oil under FCC unit conditions over different zeolite-based catalysts. Journal of Industrial and Engineering Chemistry., 2019; 78, 372–382 p. doi: 10.1016/j.jiec.2019.05.032

Klemz AC, Weschenfelder SE, Lima de Carvalho Neto SPD, et al. Oilfield produced water treatment by liquid-liquid extraction: A review. Journal of Petroleum Science and Engineering., 2021; 199, 108282 p. doi: 10.1016/j.petrol.2020.108282

Chan YH, Loh SK, Chin BLF, et al. Fractionation and extraction of bio-oil for production of greener fuel and value-added chemicals: Recent advances and future prospects. Chemical Engineering Journal., 2020; 397, 125406 p. doi: 10.1016/j.cej.2020.125406

Campos-franzani MI, Gajardo-Parra NF, Pazo-Carballo CA, et al. Extraction of guaiacol from hydrocarbons as an alternative for the upgraded bio-oil purification: Experimental and computational thermodynamic study. Fuel., 2020; 280, 118405 p. doi: 10.1016/j.fuel.2020.118405

.31. Kanaujia PK, Naik DV, Tripathi D, et al. Pyrolysis of Jatropha Curcas seed cake followed by optimization of liquid/liquid extraction procedure for the obtained bio-oil. Journal of Analytical and Applied Pyrolysis, 2016; 118, 202–224 p. doi: 10.1016/j.jaap.2016.02.005

Li H, Xia S, Ma P. Upgrading fast pyrolysis oil: Solvent–anti-solvent extraction and blending with diesel. Energy Conversion and Management., 2016 110, 378-385 p. doi: 10.1016/j.enconman.2015.11.043

Parhi SS, Rangaiah GP, Jana AK. Vapor recompressed batch distillation: Optimizing reflux ratio at variable mode. Computers & Chemical Engineering., 2019; 124, 184–196 p. doi: 10.1016/j.compchemeng.2019.02.014

Sánchez-Borrego FJ, Álvarez-Mateos P, García-Martín JF. Biodiesel and Other Value-Added Products from Bio-Oil Obtained from Agrifood Waste. Processes., 2021; 9 (5), 97 p. doi:

Li S, Zhu X, Li S, et al. Improved bio-oil distilling effect by adding additives to enhance downstream bio-oil processing and separation. Separation and Purification Technology., 2020; 247, 116982 p. doi: 10.1016/j.seppur.2020.116982

Rahman S, Heuller R, MacQuarrie S, et al. Upgrading and isolation of low molecular weight compounds from bark and softwood bio-oils through vacuum distillation. Separation and Purification Technology., 2018; 194, 123–129 p. doi: 10.1016/j.seppur.2017.11.033

Wang S, Gu Y, Liu Q, et al. Separation of bio-oil by molecular distillation. Fuel Processing Technology., 2009; 90 (5), 738–745 p. doi: 10.1016/j.fuproc.2009.02.005

Kim JS. Production, separation and applications of phenolic-rich bio-oil – A review. Bioresource Technology., 2015; 178, 90–98 p. doi: 10.1016/j.biortech.2014.08.121

William B, Noémie P, Brigitte E, et al. Supercritical fluid methods: An alternative to conventional methods to prepare liposomes. Chemical Engineering Journal, 2020; 383, 123106 p. doi: 10.1016/j.cej.2019.123106

Da Silva RPFF, Rocha-santos TAP, Duarte AC. Supercritical fluid extraction of bioactive compounds. TRAC Trends in Analytical Chemistry, 2016; 76, 40–51 p. doi: 10.1016/j.trac.2015.11.013

Molino A, Mehariya S, Di Sanzo G, et al. Recent developments in supercritical fluid extraction of bioactive compounds from microalgae: Role of key parameters, technological achievements and challenges. Journal of CO2 Utilization., 2020; 36, 196–209 p. doi: 10.1016/j.jcou.2019.11.014

Saini RK, Keum YS. Carotenoid extraction methods: A review of recent developments. Food Chemistry., 2018; 240, 90–103 p. doi: 10.1016/j.foodchem.2017.07.099

Herrero M, Cifuentes A, Ibanez E. Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae: A review. Food Chemistry., 2006; 98 (1), 136–148 p. doi: 10.1016/j.foodchem.2005.05.058

Goto M, Kanda H, Wahyudiono, et al. Extraction of carotenoids and lipids from algae by supercritical CO2 and subcritical dimethyl ether. The Journal of Supercritical Fluids., 96, 245–251 p. doi: 10.1016/j.supflu.2014.10.003

Jo H, Prajitno H, Zeb H, et al. Upgrading low-boiling-fraction fast pyrolysis bio-oil using supercritical alcohol: Understanding alcohol participation, chemical composition, and energy efficiency. Energy Conversion and Management., 2017; 148, 197–209 p. doi: 10.1016/j.enconman.2017.05.061

Montesantos N, Pedersen TH, Pedersen RD, et al. Supercritical carbon dioxide fractionation of bio-crude produced by hydrothermal liquefaction of pinewood. The Journal of Supercritical Fluids., 2019; 149, 97–109 p. doi: 10.1016/j.supflu.2019.04.001

Park JY, Jeon W, Lee JH, et al. Effects of supercritical fluids in catalytic upgrading of biomass pyrolysis oil. Chemical Engineering Journal., 2019; 377, 120312 p. doi: 10.1016/j.cej.2018.11.010

Ahamed TS, Anto S, Mathimani T, et al. Upgrading of bio-oil from thermochemical conversion of various biomass – Mechanism, challenges and opportunities. Fuel., 2021; 287, 119329 p. doi: 10.1016/j.fuel.2020.119329

Wang S, Chen J, Cai Q, et al. The effect of mild hydrogenation on the catalytic cracking of bio-oil for aromatic hydrocarbon production. International Journal of Hydrogen Energy., 2016; 41 (37), 16385–16393 p. doi: 10.1016/j.ijhydene.2015.12.024

Lian X, Xue Y, Zhao Z, et al. Progress on upgrading methods of bio-oil: A review. International Journal of Energy Research., 2017; 41 (13), 1798–1816 p. doi: 10.1002/er.3726

Gueudré L, Thegarid N, Burel L, et al. Coke chemistry under vacuum gasoil/bio-oil FCC co-processing conditions. Catalysis Today., 2015; 257, 200–212 p. doi: 10.1016/j.cattod.2014.09.001

Chew TL, Bhatia S. Effect of catalyst additives on the production of biofuels from palm oil cracking in a transport riser reactor. Bioresource Technology., 2009; 100 (9), 2540–2545 p. doi: 10.1016/j.biortech.2008.12.021

Chen G, Zhang R, Ma W, et al. Catalytic cracking of model compounds of bio-oil over HZSM-5 and the catalyst deactivation. Science of The Total Environment., 2018; 631-632, 1611–1622 p. doi: 10.1016/j.scitotenv.2018.03.147

Dotto GL, Mckay G. Current scenario and challenges in adsorption for water treatment. Journal of environmental chemical engineering., 2020; 8 (4), 103988 p. doi:

Xu X, Li Z, Sun Y, et al. High-Quality Fuel from the Upgrading of Heavy Bio-oil by the Combination of Ultrasonic Treatment and Mutual Solvent. Energy e fuels., 2018; 32, 3477-3487 p. doi: 10.1021/acs.energyfuels.7b03483

Widjaja C, Yovita D, Alfin K, et al. Biorefinery concept on jackfruit peel waste: Bio-oil upgrading. Journal of Engineering and Applied Sciences., 2018; 13, 2202-2207 p. available at

Zhang M, Shen Q, Wu H. Adsorption Characteristics of Bio-oil on Biochar in Bioslurry Fuels. Energy and Fuels., 2017; 31 (9), 9619–9626 p. doi: 10.1021/acs.energyfuels.7b02041

Das P, Ganesh A. Bio-oil from pyrolysis of cashew nut shell—a near fuel. Biomass and Bioenergy., 2003; 25(1), 113–117 p. doi: 10.1016/s0961-9534(02)00182-4

Resasco DE, Crossley SP. Implementation of concepts derived from model compound studies in the separation and conversion of bio-oil to fuel. Catalysis Today., 2015; 257, 185–199 p. doi: 10.1016/j.cattod.2014.06.037

Xun H, Zhanming Z, Mortaza G, et al. Coke formation during thermal treatment of bio-oil. Energia e Combustíveis., 2020; 34 (7), 7863-7914 p. doi: 10.1021/acs.energyfuels.0c01323

Wang S, Li Z, Yi W, et al. Catalytic pyrolysis of maize cob lignin over activated red mud catalyst for value-added mono-phenol production. Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering., 2020; 36 (13), 203-211 p. doi: 10.11975/j.issn.1002-6819.2020.13.024

Zhang, JJR, Hainian W. Modification Mechanism of Using Waste Wood–Based Bio-Oil to Modify Petroleum Asphalt. Journal of Materials in Civil Engineering.,2020; 32, 12p. doi:10.1061/(ASCE)MT.1943-5533.0003464

Mashuni R, H, Hamid FH, Widiyani M, et al. Analysis of bio-oil effectiveness from coconut shells pyrolysis as biopesticide by potentiometric biosensor. Journal of Physics: Conference Series., 2021; 1825(1), 012095 p. doi: 10.1088/1742-6596/1825/1/012095

Czernik S, Bridgwater AV. Overview of applications of biomass fast pyrolysis oil. Energy & fuels., 2004; 18(2), 590-598p. doi: 10.1021/ef034067u

Hu X, Gholizadeh M. Biomass pyrolysis: A review of the process development and challenges from initial researches up to the commercialization stage. Journal of Energy Chemistry., 2019; 39, 109–143 p. doi: 10.1016/j.jechem.2019.01.024

Huang Y, Li Y, Han X, et al. Investigation on fuel properties and engine performance of the extraction phase liquid of bio-oil/biodiesel blends. Renewable Energy., 2020; 147, 1990-2002p. doi: 10.1016/j.renene.2019.10.028

Mariappan M, Panithasan MS, Venkadesan G. Pyrolysis plastic oil production and optimisation followed by maximum possible replacement of diesel with bio-oil/methanol blends in a CRDI engine. Journal of Cleaner Production., 2021; 312, 127687 p. doi: 10.1016/j.jclepro.2021.127687

Podrojková N, Oriňak A, Oriňaková R, et al. Effect of different crystalline phase of ZnO/Cu nanocatalysts on cellulose pyrolysis conversion to specific chemical compounds. Cellulose., 2018; 25(10), 5623–5642 p. doi: 10.1007/s10570-018-1997-7

Chen Y, Cao X, Zhu S, et al. Synergistic hydrothermal liquefaction of wheat stalk with homogeneous and heterogeneous catalyst at low temperature. Bioresource Technology, 2019; 278, 92–98 p. doi: 10.1016/j.biortech.2019.01.076

Li H, Mahmood N, Ma Z, et al. Preparation and characterization of bio-polyol and bio-based flexible polyurethane foams from fast pyrolysis of wheat straw. Industrial Crops and Products., 2017; 103, 64–72 p. doi: 10.1016/j.indcrop.2017.03.042

Fardhyanti DS, Kadarwati S, Dewajani H, et al. Modelling of Liquid-Liquid Equilibria Using NRTL and UNIFAC Equations in the Extraction of Bio-Oil-Based Phenolics Produced from the Pyrolysis of Sugarcane Bagasse. Materials Science Forum., 2022; 1048, 445–450 p. doi: 10.4028/

Li Shi Y, Zhu X, Zhang L, et al. Atmospheric distillation of bio-oil based on different extractants. Ranliao Huaxue Xuebao/Journal of Fuel Chemistry and Technology., 2019; 47(3), 312-317 p. available at

Geng F, Zhang R, Liu H, et al. Research progress on separation of bio-oil components and chemical extraction. Progress in Chemical Industry., 2021; 40(12), 2020-2539 p. doi: 10.16085/j.issn.1000-6613

Han Y, Pires APP, Denson M, et al. Ternary Phase Diagram of Water/Bio-Oil/Organic Solvent for Bio-Oil Fractionation. Energy & Fuels, 2020; 34(12), 16250-16264 p. doi: 10.1021/acs.energyfuels.0c03100

Mathanker A, Das S, Pudasainee D, et al. A Review of Hydrothermal Liquefaction of Biomass for Biofuels Production with a Special Focus on the Effect of Process Parameters, Co-Solvents, and Extraction Solvents. Energies, 2021; 14(16), 4916 p. doi: 10.3390/en14164916

Aljaziri J, Gautam R, Alturkistani S, et al. On the effects of CO2 atmosphere in the pyrolysis of Salicornia bigelovii. Bioresource Technology Reports., 2022; 17, 100950 p. doi: 10.1016/j.biteb.2022.100950

Zhang XS, Yang GX, Jiang H, et al. Mass production of chemicals from biomass-derived oil by directly atmospheric distillation coupled with co-pyrolysis. Scientific Reports., 2013; 3(1), 1-7 p. doi: 10.1038/srep01120

Lv S, Yuan J, Peng X, et al. Performance and optimization of bio-oil/Buton rock asphalt composite modified asphalt. Construction and Building Materials., 2020; 264, 120235 p. doi: 10.1016/j.conbuildmat

Zhang R, Wang H, Jiang X, et al. Thermal storage stability of bio-oil modified asphalt. J. Mater. Civ. Eng., 2018; 30(4), 04018054p. doi: 10.1061/(ASCE)MT.1943-5533.0002237

Urrutia RI, Gutierrez VS, Stefanazzi N, et al. Pyrolysis liquids from lignocellulosic biomass as a potential tool for insect pest management: A comprehensive review. Industrial Crops and Products, 2022; 177, 114533 p. doi: 10.1016/j.indcrop.2022.114533

Bolzan A, Padovez FEO, Nascimento ARB, et al. Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross-resistance to other diamide insecticides. Pest Management Science, 2019; 75(10), 2682–2689 p. doi: 10.1002/ps.5376

Mattos C, Veloso MCC, Romeiro GA, et al. Biocidal applications trends of bio-oils from pyrolysis: Characterization of several conditions and biomass, a review. Journal of Analytical and Applied Pyrolysis, 2019; 139, 1–12 p. doi: 10.1016/j.jaap.2018.12.029

Yatagai M, Nishimoto M, Hori K, et al. Termiticidal activity of wood vinegar, its components and their homologues. Journal of Wood Science., 2002; 48(4), 338–342 p. doi: 10.1007/BF00831357

Kiarie-Makara MW, Yoon HS, Lee DK. Repellent efficacy of wood vinegar against Culex pipiens pallens and Aedes togoi (Diptera: Culicidae) under laboratory and semi-field conditions. Entomological Research., 2010; 40(2), 97–103 p. doi: 10.1111/j.1748-5967.2010.00265x

Wagiman FX, Ardiansyah A, Witjaksono et al. Activity of coconut-shell liquid-smoke as an insecticide on the rice brown planthopper (Nilaparvata lugens). Journal of Agricultural and Biological Science, 2014; 9(9), 293-296 p. available at

Mohan D, Shi J, Nicholas DD, et al. Fungicidal values of bio-oils and their lignin-rich fractions obtained from wood/bark fast pyrolysis. Chemosphere., 2008; 71(3), 456–465 p. doi: 10.1016/j.chemosphere.2007.10.049

Kartal SN, Terzi E, Kose C, et al. Efficacy of tar oil recovered during slow pyrolysis of macadamia nut shells. International Biodeterioration & Biodegradation, 2011; 65 (2), 369-373 p. doi: 10.1016/j.ibiod.2010.08.011



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